Anti-CD1d [NOR3.2 (NOR3.2/13.17)] antibody (ab11076)

Overview

• Product nameAnti-CD1d [NOR3.2 (NOR3.2/13.17)] antibodySee all CD1d primary antibodies ...
• Description
  Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
• Tested applicationsICC/IF, IHC-Fr, IHC-P, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Sheep, Human
  
• Immunogen

  Beta G2 - CD1d fusion protein (Human).

• Positive controlThis antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.4
  Preservative: 0.02% Sodium azide
  Constituent: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurified from tissue culture supernatant.
• ClonalityMonoclonal
• Clone numberNOR3.2 (NOR3.2/13.17)
• MyelomaNS0
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • NK Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Dendritic Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Mast Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Immunoglobulins
  • Receptors

Applications

Target

• FunctionAntigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
• Tissue specificityExpressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
• Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
• Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.

Target information above from: UniProt accession P15813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 912 Human
  • Omim: 188410 Human
  • SwissProt: P15813 Human
  • Unigene: 1799 Human

• Alternative names
  Antigen-presenting glycoprotein CD1d antibodyCD1.1 antibodyCD1A antibody

  CD1d antibodyCD1D antigen antibodyCD1D antigen d polypeptide antibodyCD1d molecule antibodyCD1D_HUMAN antibodyCd1d1 antibodyDifferentiation antigen CD1 alpha 3 antibodyHMC class I antigen like glycoprotein CD1D antibodyLy 38 antibodyMGC34622 antibodyR3 antibodyR3G1 antibodyT cell surface glycoprotein CD1d antibodyT cell surface glycoprotein CD1d precursor antibodyThymocyte antigen CD1D antibody

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Anti-CD1d [NOR3.2 (NOR3.2/13.17)] antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

  IHC image of CD1d staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab11076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

  Overlay histogram showing MOLT4 cells stained with ab11076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11076, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight&reg; 488 goat anti-mouse IgG (H+L) (<ab96879>ab96879</ab>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (<ab91353>ab91353</ab>, 2µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Image courtesy of an anonymous Abreview.
  ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.

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