![Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076) image](#ab11076_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076) image](#CD1d-Primary-antibodies-ab11076-13.jpg)
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Product name
Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]
See all CD1d products (14) ...
Description
Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
Tested applications
ICC/IF, IHC-Fr, IHC-P, IP, Flow Cytmore details
Cross reactivity
Reacts with
Sheep, Human
Immunogen
Beta G2 - CD1d fusion protein (Human).
Positive control
Skin, tonsil.
General notes
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from tissue culture supernatant.
Clonality
Monoclonal
Clone number
NOR3.2 (NOR3.2/13.17)
Myeloma
NS0
Isotype
IgG1
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Immunoglobulins >> Receptors
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Tissue specificity
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
Target information above from: UniProt accessionP15813
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen-presenting glycoprotein CD1d antibody
- CD1.1 antibody
- CD1A antibody
see all
Database links
- Entrez Gene: 912 Human
- Omim: 188410 Human
- SwissProt: P15813 Human
- Unigene: 1799 Human
Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] images:
Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076)
![Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076)](/ps/datasheet/Images/11/ab11076/ab11076_1.jpg)
ab11076 staining CD1d in MOLT4 cells by Flow Cytometry analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)](/ps/datasheet/images/11/ab11076/CD1d-Primary-antibodies-ab11076-13.jpg)
ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.
Image courtesy of an anonymous Abreview.
References for Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
This product has been referenced in:
- Allen CE et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr; Human.Read more (PubMed: 20220088) »
- Xu C et al. Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol 85:539-41 (2010). IHC.Read more (PubMed: 20575029) »
See all 5 publications for this product
Publishing research using ab11076? Please let us know so that we can cite the reference in this datasheet
![Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (11076)](/ps/datasheet/images/11/ab11076/CD1d-Primary-antibodies-ab11076-11.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"