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Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

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Overview

Product name

Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]
See all CD1d products (14) ...

Description

Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d

Tested applications

ICC/IF, IHC-Fr, IHC-P, IP, Flow Cytmore details

Cross reactivity

Reacts with

Sheep, Human

Immunogen

Beta G2 - CD1d fusion protein (Human).

Positive control

Skin, tonsil.

General notes

Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified from tissue culture supernatant.

Clonality

Monoclonal

Clone number

NOR3.2 (NOR3.2/13.17)

Myeloma

NS0

Isotype

IgG1

  • Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076)Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

Tissue specificity

Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Cellular localization

Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.

Target information above from: UniProt accessionP15813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Antigen-presenting glycoprotein CD1d antibody
  • CD1.1 antibody
  • CD1A antibody
  • Cd1d antibody
  • CD1D antigen antibody
  • CD1D antigen d polypeptide antibody
  • CD1d molecule antibody
  • CD1D_HUMAN antibody
  • Cd1d1 antibody
  • Differentiation antigen CD1 alpha 3 antibody
  • HMC class I antigen like glycoprotein CD1D antibody
  • Ly 38 antibody
  • MGC34622 antibody
  • R3 antibody
  • R3G1 antibody
  • T cell surface glycoprotein CD1d antibody
  • T cell surface glycoprotein CD1d precursor antibody
  • Thymocyte antigen CD1D antibody
see all

Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] images:

  Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076)

Flow Cytometry - CD1d antibody [ NOR3.2 (NOR3.2/13.17) ] (ab11076)

ab11076 staining CD1d in MOLT4 cells by Flow Cytometry analysis.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

This product has been referenced in:

  • Allen CE  et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr; Human.Read more (PubMed: 20220088) »
  • Xu C  et al. Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol 85:539-41 (2010). IHC.Read more (PubMed: 20575029) »

See all 5 publications for this product

Publishing research using ab11076? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"