Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [EP459Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Sample
Human Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/1000
Username

Abcam user community

Verified customer

Submitted Apr 06 2015

Sign up