![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD20 antibody [L26] (ab9475) image](#ab9475_1.jpg)
![Western blot - CD20 antibody [L26] (ab9475) image](#CD20-Primary-antibodies-ab9475-3.jpg)
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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD20 antibody [L26]
See all CD20 products (45) ...
Description
Mouse monoclonal [L26] to CD20
Specificity
This antibody is specific to a 33 kDa polypeptide present on the majority of B cells in peripheral blood and lymphoid tissue and also with a minor component of 30 kDa. No reactivity with other haematopoietic cells has been observed.
Tested applications
WB, IP, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Human tonsil B cells.
Positive control
Tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
L26
Myeloma
unknown
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/750.Predicted molecular weight: 33 kDa.
IP
IP: 1/100 - 1/750.IP: 1/100 - 1/750.
IHC-P: 1/20 - 1/50.
IHC-Fr
IHC-Fr: 1/20 - 1/50.IHC-Fr: 1/20 - 1/50.
Target
Function
This protein may be involved in the regulation of B-cell activation and proliferation.
Tissue specificity
Expressed on B-cells.
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similarities
Belongs to the MS4A family.
Post-translational
modifications
Phosphorylated. Might be functionally regulated by protein kinase(s).
Cellular localization
Membrane.
Target information above from: UniProt accessionP11836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
Anti-CD20 antibody [L26] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD20 antibody [L26] (ab9475)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD20 antibody [L26] (ab9475)](/ps/datasheet/Images/9/ab9475/ab9475_1.jpg)
ab9475 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with CD20, B cell using ABC and Fast Red chromagen.
Western blot - CD20 antibody [L26] (ab9475)
![Western blot - CD20 antibody [L26] (ab9475)](/ps/datasheet/images/9/ab9475/CD20-Primary-antibodies-ab9475-3.jpg)
Predicted band size : 33 kDa
CD20 associates with phosphorylated proteins after BCR stimulation. Ramos cells were unstimulated or stimulated with F(ab′)2 anti-Igμ for the times and temperatures indicated. Cells were lysed in digitonin, and CD20 was Immunoprecipitated from cleared lysates. Immunoprecipitates were probed using anti-phosphotyrosine (ptyr) (n = 4). The membranes were stripped and reprobed for CD20 using ab9475 (lower panel).
Image from Polyak MJ et al, J Biol Chem 283:18545-52 (2008), Fig 5.
References for Anti-CD20 antibody [L26] (ab9475)
This product has been referenced in:
- Dalle Set al. Preclinical studies on the mechanism of action and the anti-lymphoma activity of the novel anti-CD20 antibody GA101. Mol Cancer Ther 10:178-85 (2011).Read more (PubMed: 21220500) »
- Verneuil Let al. Endothelial damage in all types of T-lymphocyte-mediated drug-induced eruptions. Arch Dermatol 147:579-84 (2011).Read more (PubMed: 21576578) »
See all 8 publications for this product
Publishing research using ab9475? Please let us know so that we can cite the reference in this datasheet
![CD20 antibody [L26] for IHC-P in Human (9475)](/ps/Reviews/Images/ab5274_17592.jpg)
![CD20 antibody [L26] for IHC-P in Human (9475)](/ps/Reviews/Images/ab12096_66087.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"