CD21 Human ELISA Kit (ab45909)

Overview

• Product nameCD21 Human ELISA KitSee all CD21 kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Sample A	8	50.25U/ml	2.24	4.45%
  Sample B	8	10.44U/ml	0.46	4.4%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Sample A	8	51.13U/ml	2.29	4.47%
  Sample B	8	10.76U/ml	0.56	5.2%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 3 U/ml
• Range
  3.12 U/ml - 100 U/ml
• Assay time
  1h 45m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The Human sCD21 ELISA kit is to be used for the in-vitro quantitative determination of the molecule in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize natural human sCD21. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The sCD21 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD21 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known sCD21 concentrations are pipetted into these wells.

  During the first incubation, the sCD21 antigen and a biotinylated monoclonal antibody specific for CD21 are simultaneously incubated.

  After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sCD21 present in the samples.

   

  Recognizes natural soluble CD21
  
  Range: 3.12 U/ml - 100 U/ml
  
  Sensitivity <3 U/ml
  

• Notes

  Safety Information:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

Components

Kit Components	Unit Size/Volume
96-well microtiter plates	2
Plastic cover	4
Standard : 100 U/ml	4 vials
Standard Diluent Buffer	1 vial
Biotinylated anti-sCD21	2 vials
Biotinylated Antibody Diluent	1 vial (13 ml
Streptavidin HRP	4 vials
HRP Diluent	1 vial
Washing Buffer	2 vials
Chromogen TMB	1 vial (24 ml)
H2SO4 : Stop Reagent	2 vials

Note: For more detailed instructions view specific protocol
• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • CD markers ELISA kits

  • Immunology
  • Adaptive Immunity
  • B Cells
  • CD

Applications

CD21 Human ELISA Kit images

• 

  ELISA - Human CD21 ELISA kit (ab45909)
  Typical sCD21 standard curve ranging from 3.12 to 100U/mL

Protocols

• Human CD21 ELISA kit