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Overview

  • Product nameAnti-CD21 antibody [EP3093]
    See all CD21 primary antibodies
  • Description
    Rabbit monoclonal [EP3093] to CD21
  • Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human CD21

  • Positive control
    • Daudi cell lysate, Raji cell lysate and Tonsil tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Anti-CD21 antibody (HRP) [EP3093] (ab202354)

    Anti-CD21 antibody [EP3093] (DyLight® 488) (ab139873)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP3093
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 113 kDa).
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionReceptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
  • Tissue specificityMature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
  • Involvement in diseaseGenetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3DR antibody
    • CD 21 antibody
    • CD21 antibody
    • Complement C3d receptor antibody
    • Complement component (3d/Epstein Barr virus) receptor 2 antibody
    • Complement receptor type 2 antibody
    • CR antibody
    • Cr2 antibody
    • CR2_HUMAN antibody
    • CVID7 antibody
    • EBV receptor antibody
    • EBV-R antibody
    • Epstein Barr virus receptor antibody
    • Epstein-Barr virus receptor antibody
    • EVBR antibody
    • SLEB9 antibody
    see all

Anti-CD21 antibody [EP3093] images

  • All lanes : Anti-CD21 antibody [EP3093] (ab75985) at 1/2000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 113 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin-embedded human tonsils using ab75985 at 1/250-1/500 dilution.
  • ab75985 showing positive staining in Normal tonsil tissue.

  • ab75985 showing negative staining in Normal liver tissue.

  • ab75985 showing negative staining in Normal kidney tissue.

  • ab75985 staining CD21 in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide, fixed in paraformaldehyde and incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Live Lymphocytes.

    See Abreview

  • ab75985 showing negative staining in Normal brain tissue.

  • ab75985 showing negative staining in Normal breast tissue.

  • ab75985 showing positive staining in Normal tonsil tissue.

  • ab75985 showing negative staining in Normal brain tissue.

  • ab75985 showing negative staining in Normal liver tissue.

  • ab75985 showing negative staining in Normal kidney tissue.

  • ab75985 showing negative staining in Normal breast tissue.

References for Anti-CD21 antibody [EP3093] (ab75985)

This product has been referenced in:
  • Di Caro G  et al. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Clin Cancer Res 20:2147-58 (2014). Read more (PubMed: 24523438) »
  • Baik J  et al. Multiscale distribution and bioaccumulation analysis of clofazimine reveals a massive immune system-mediated xenobiotic sequestration response. Antimicrob Agents Chemother 57:1218-30 (2013). IHC ; Mouse . Read more (PubMed: 23263006) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: form buffy coat after FICOLL
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy lymphocyte gated
Username

Abcam user community

Verified customer

Submitted Nov 01 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Thank you for submitting an Abreview of ab75985. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

Read More
Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: after spleen mashing
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 18 2012

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen from immune mice)
Specification spleen from immune mice
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

Yes, the epitope of this antibody is intracellular. Therefore it will be necessary to permeabilize the cells for flow cytometry.

Thank you for contacting Abcam. I can provide the broader range of the immunogen for the blocking peptide. The peptide is derived from the terminal region, amino acid ***********of human CD21 (UniProt: P20023). I am sorry that I am unable to provide a ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"