Anti-CD229 antibody (ab113285)
Key features and details
- Rabbit polyclonal to CD229
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD229 antibody
See all CD229 primary antibodies -
Description
Rabbit polyclonal to CD229 -
Host species
Rabbit -
Specificity
At least three isoforms are known to exist. ab113285 is predicted to not cross-react with other family members. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
An 18 amino acid peptide near the C terminus of Human CD229.
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Positive control
- 293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab113285 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa.
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ICC |
1/10.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa. |
ICC
1/10. |
Target
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Function
May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4063 Human
- Omim: 600684 Human
- SwissProt: Q9HBG7 Human
- Unigene: 403857 Human
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Alternative names
- CD229 antibody
- Cell surface molecule Ly-9 antibody
- Cell surface molecule Ly9 antibody
see all
Images
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Lane 1 : Anti-CD229 antibody (ab113285) at 1 µg/ml
Lane 2 : Anti-CD229 antibody (ab113285) at 2 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg/ml per lane.
Predicted band size: 72 kDa -
Immunofluorescence of CD229 in 293 cells with ab113285 at 20 ug/mL.
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Immunocytochemical analysis of 293 cells, staining CD229 with ab113285 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113285 has not yet been referenced specifically in any publications.