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Product Name
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CD24 antibody [8.B.76]
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See all CD24 antibodies (21)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [8.B.76] to CD24
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Immunogen
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Human bone marrow malignant cells from a non-B, non-T acute leukemia
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use 2µg for 105 cells.
ICC (Cell smear) : 1/25 - 1/50.
IHC-P: Use at an assay dependent dilution (PMID 19696931).
IHC-Fr: 1/25 - 1/50.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane, attached by a GPI anchor.
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Research areas
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Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Tumor antigens
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer
ab31622 binds to the non-glycosylated GPI anchor of the protein core of the cluster w4/CD24. It recognizes B cells from pre-B stage to mature-B stage, the majority of normal medullar granulous cells, peripheral blood polymorphonuclear cells, non-T, non-B acute lymphoblastic leukemias, some atypic or myeloid leukemias or B chronic lymphocytic leukemias and two-thirds of Epstein-Barr virus transformed cell lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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8.B.76
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Myeloma
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NS1/1-Ag4-1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 40% Glycerol, PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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Ammonium sulfate fraction and column chromatography.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD24 antibody [8.B.76] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for CD24 antibody [8.B.76] (ab31622)
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This product has been used in:
Metsuyanim S et al. Expression of stem cell markers in the human fetal kidney. PLoS One 4:e6709 (2009). IHC-P; Human. PubMed: 19696931
If you publish research using ab31622 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD24
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