|
|
|
|
Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)
![Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)](/ps/datasheet/images/ab25657.png)
(enlarge)
|
|
|
|
|
Product Name
|
CD24 antibody [91] (FITC)
|
| |
See all CD24 antibodies (21)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rat monoclonal [91] to CD24 (FITC)
|
|
Immunogen
|
The details of the immunogen for this antibody are not available.
|
|
Reacts with
(species key)
|
Ms
|
|
Specificity
|
ab25657 recognises mouse heat stable antigen (HSA).
|
|
Tested applications
(see key)
|
Flow Cyt, IHC-Fr, IP
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions Flow Cyt: Use 4µg for 106 cells. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cell Membrane, attached by a GPI anchor.
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Cellular metabolic process Cancer >> Cancer Metabolism >> Response to hypoxia Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage Cancer >> Tumor biomarkers >> Tumor antigens Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative Immunology >> Adaptive Immunity >> B Cells >> CD Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
|
|
Relevance
|
CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer
This antibody can be used to inhibit B-cell differentiation.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 12484 (Mouse)
|
| |
|
|
Raised in
|
Rat
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
91
|
|
Isotype
|
IgG2a
|
|
Light chain type
|
kappa
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Conjugation
|
Fluorescein
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C in the dark.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD24 antibody [91] (FITC) is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for CD24 antibody [91] (FITC) images, references, products related to ab25657 and other tools.
|
| |
CD24 antibody [91] (FITC) images:
|
Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)
This FACS image shows BALB/c spleen cells double-stained with rat anti-mouse CD24-FITC[ab25657 at 3µg/million cells] and hamster anti-mouse CD3epsilon-RPE.
This FACS image shows BALB/c spleen cells double-stained with ab25657 at 3µg/106 cells and hamster anti-mouse CD3epsilon-RPE. Cells were then gated on small lymphocytes and analyzed using a FACScanTM flow cytometer (BDIS, San Jose, CA).
|
| |
| |
|
Search PubMed (MEDLINE) for references to CD24
|
| |
CD24 antibody [91] (FITC) - more information
|
Customer reviews (feedback) regarding CD24 antibody [91] (FITC) |
Customer FAQs regarding CD24 antibody [91] (FITC) |
Protocols for CD24 antibody [91] (FITC) |
Price and availability of products related to CD24 antibody [91] (FITC) |
| |
CD24 antibody [91] (FITC) - related products:
|
|
|
| |
CD24 antibody [91] (FITC) - other tools:
|
Contact Abcam with a Technical Enquiry about CD24 antibody [91] (FITC) |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"