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Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)
Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)
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Product Name 

CD24 antibody [91] (FITC)

 

See all CD24 antibodies (21)...

Product type 

Primary antibodies

Description 

Rat monoclonal [91] to CD24 (FITC)

Immunogen 

The details of the immunogen for this antibody are not available.

Reacts with 
(species key)

Ms

Specificity 

ab25657 recognises mouse heat stable antigen (HSA).

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IP


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 4µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane, attached by a GPI anchor.

Research areas 

Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Tumor antigens
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer


This antibody can be used to inhibit B-cell differentiation.

 

Alternative names CD24 antibody [91] (FITC) (ab25657)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

12484    (Mouse)

SwissProt

    

P24807    (Mouse)

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

91

Isotype 

IgG2a

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C in the dark.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD24 antibody [91] (FITC) is on this datasheet. But please do contact us if you would like any reassurance!


See below for CD24 antibody [91] (FITC) images, references, products related to ab25657 and other tools.

 

CD24 antibody [91] (FITC) images:

Product image  Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)

This FACS image shows BALB/c spleen cells double-stained with rat anti-mouse CD24-FITC[ab25657 at 3µg/million cells] and hamster anti-mouse CD3epsilon-RPE.

Flow Cytometry - CD24 antibody [91] (FITC) (ab25657)

This FACS image shows BALB/c spleen cells double-stained with ab25657 at 3µg/106 cells and hamster anti-mouse CD3epsilon-RPE.  Cells were then gated on small lymphocytes and analyzed using a FACScanTM flow cytometer (BDIS, San Jose, CA).

 
 

Search PubMed (MEDLINE) for references to CD24

 

CD24 antibody [91] (FITC) - more information

Customer reviews (feedback) regarding CD24 antibody [91] (FITC)

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Price and availability of products related to CD24 antibody [91] (FITC)

 

CD24 antibody [91] (FITC) - related products:

 

Compatible Secondaries for CD24 antibody [91] (FITC)

ab6565

  

Cy5 ® antibody

ab6730

  

FITC antibody

ab6733

  

Biotin antibody

ab6734

  

HRP antibody

ab6843

  

Texas Red ® antibody

ab6846

  

Alkaline Phosphatase antibody

ab7010

  

Phycoerythrin antibody

ab7020

  

Agarose antibody

 

Other CD24 Antibodies

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CD24 antibody [91] (FITC) - other tools:

Contact Abcam with a Technical Enquiry about CD24 antibody [91] (FITC)

 

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