CD24 antibody [ALB9] (ab19704)

Product Name 

CD24 antibody [ALB9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ALB9] to CD24

Immunogen 

Bone marrow malignant cells from a non-B, non-T acute leukemia.

Reacts with 
(species key)

Hu

Specificity 

Recognizes B cells from pre-B to mature-B stage. Studies have shown that CD24 recognizes the majority of normal medullar granulous cells and peripheral blood polymorphonuclear cells.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 2µg for 5x 10⁵ cells (see Duperray et al).
ICC: 1/25 - 1/50.
IHC-Fr: 1/25 - 1/50 (see Boucheix et al).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane, attached by a GPI anchor.

Research areas 

Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Tumor antigens
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ALB9

Myeloma 

NS1/1-Ag4-1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstitution form for up to one month at 2-8°C.
Constituents: PBS, 1mg/ml BSA

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CD24 antibody [ALB9] (ab19704)

  This product has been used in: (two most recent references)

Ye P et al. CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biol 10:2 (2009). PubMed: 19138432

This clone has been used in:

See all 6 publication references for this product.


If you publish research using ab19704 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding CD24 antibody [ALB9]

Customer FAQs regarding CD24 antibody [ALB9]

Protocols for CD24 antibody [ALB9]

Price and availability of products related to CD24 antibody [ALB9]

CD24 antibody [ALB9] - related products: