CD26 Human ELISA Kit (with color giving dyes) (ab119513)
- Product nameCD26 Human ELISA Kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Overall 4.6% Inter-assay Sample n Mean SD CV% Overall 9.1%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity7.3 ng/ml
- Range15.6 ng/ml - 500 ng/ml
Sample specific recovery Sample type Average % Range Serum 101 81% - 116%
- Species reactivityReacts with: Human
- Product overview
ab119513 CD26 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD26. ab119513 is for research use only. Not for diagnostic or therapeutic procedures.
CD26, a T cell activation antigen, is a 110kDa cell surface glycoprotein with known dipeptidyl peptidase IV activity in its extracellular domain which is present on various cell types, including T cells and epithelial cells of the liver, kidney, and intestine. Of the T cell antigens described to date, CD26 has proved to be one of the most intriguing in the scope of its functional associations. Considerable evidence suggests that CD26 can deliver a potent co-stimulatory signal to T-cells. This signal transducing property appears to be a property of its extracellular domain. In addition, CD26 appears to be a functional collagen receptor that may aid activated T-cells in localizing to inflammatory regions. It has also been demonstrated that CD26 not only acts as a functional dipeptidyl peptidase IV, but also binds strongly to adenosine deaminase.
Significant levels of DPPIV activity have been shown to exist in plasma, serum, and urine. The serum form of DPPIV is unique, and is not a breakdown product of membrane CD26, nevertheless exposing significant structural similarity to the membrane form.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human sCD26 monoclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human sCD26 Standard 2 vials Monoclonal anti-human sCD26-coated microwell plates 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
modificationsThe soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
- Adenosine deaminase complexing protein 2CD26Dipeptidyl peptidase 4Dipeptidyl peptidase 4 soluble formDipeptidyl peptidase IVDipeptidyl peptidase IV membrane formDipeptidyl peptidase IV soluble formDipeptidylpeptidase 4DPP 4DPP IVDPP4DPP4_HUMANDPPIVT-cell activation antigen CD26TP103
Our Abpromise guarantee covers the use of ab119513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD26 Human ELISA Kit (with color giving dyes) (ab119513)
ab119513 has not yet been referenced specifically in any publications.