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CD26 Human ELISA Kit (with color giving dyes) (ab119513)

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Overview

  • Product nameCD26 Human ELISA Kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.1%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    7.3 ng/ml
  • Range
    15.6 ng/ml - 500 ng/ml
  • Recovery

    101 %

    Sample specific recovery
    Sample type Average % Range
    Serum 101 81% - 116%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119513 CD26 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD26. ab119513 is for research use only. Not for diagnostic or therapeutic procedures.

    CD26, a T cell activation antigen, is a 110kDa cell surface glycoprotein with known dipeptidyl peptidase IV activity in its extracellular domain which is present on various cell types, including T cells and epithelial cells of the liver, kidney, and intestine. Of the T cell antigens described to date, CD26 has proved to be one of the most intriguing in the scope of its functional associations. Considerable evidence suggests that CD26 can deliver a potent co-stimulatory signal to T-cells. This signal transducing property appears to be a property of its extracellular domain. In addition, CD26 appears to be a functional collagen receptor that may aid activated T-cells in localizing to inflammatory regions. It has also been demonstrated that CD26 not only acts as a functional dipeptidyl peptidase IV, but also binds strongly to adenosine deaminase.

    Significant levels of DPPIV activity have been shown to exist in plasma, serum, and urine. The serum form of DPPIV is unique, and is not a breakdown product of membrane CD26, nevertheless exposing significant structural similarity to the membrane form.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionCell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificityExpressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similaritiesBelongs to the peptidase S9B family. DPPIV subfamily.
  • DomainThe extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localizationCell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.

    • Target information above from: UniProt accession P27487 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        ADABPADCP-2ADCP2
        Adenosine deaminase complexing protein 2CD26Dipeptidyl peptidase 4Dipeptidyl peptidase 4 soluble formDipeptidyl peptidase IVDipeptidyl peptidase IV membrane formDipeptidyl peptidase IV soluble formDipeptidylpeptidase 4DPP 4DPP IVDPP4DPP4_HUMANDPPIVT-cell activation antigen CD26TP103
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119513 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD26 Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - CD26 Human ELISA Kit (with color giving dyes) (ab119513)
        Sandwich ELISA - CD26 Human ELISA Kit (with color giving dyes) (ab119513)
        Representative standard curve using ab119513.

      Protocols

      References for CD26 Human ELISA Kit (with color giving dyes) (ab119513)

      ab119513 has not yet been referenced specifically in any publications.

      Publishing research using ab119513 ? Please let us know so that we can cite the reference in this datasheet

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      Dears,
      Does our kitCD26 Human ELISA Kit (with color giving dyes) (ab119513), is the same of http://www.....dppiv-drug-discovery-kit/?
      And if there is differences what they are -- urgent please.
      Thanks !

      Thank you for contacting us.

      I am afraid but our CD26 Human ELISA Kit (with color giving dyes) ab119513 is completely different from the DPPIV drug discovery kit, and the decision for either product depends on what you would like to investigate:...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"