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Overview

  • Product nameAnti-CD26 antibody [OX-61] (FITC)See all CD26 primary antibodies ...
  • Description
    Mouse monoclonal [OX-61] to CD26 (FITC)
  • ConjugationFITC
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Thoracic duct lymphocytes from a mesenteric lymphodectomised Rat.

  • Positive controlRat Strain: WistAr (Thymus, Spleen)

Properties

Applications

Our Abpromise guarantee covers the use of ab112227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use 0.5µg for 106 cells.

Target

  • FunctionCell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificityExpressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similaritiesBelongs to the peptidase S9B family. DPPIV subfamily.
  • DomainThe extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localizationCell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.

    • Target information above from: UniProt accession P27487 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ADA-binding protein antibodyADABP antibodyADCP 2 antibody
        ADCP-2 antibodyADCP2 antibodyAdenosine deaminase complexing protein 2 antibodyCD 26 antibodyCD26 antibodyCD26 antigen 3 antibodyDipeptidyl peptidase 4 antibodyDipeptidyl peptidase 4 soluble form antibodyDipeptidyl peptidase IV antibodyDipeptidyl peptidase IV membrane form antibodyDipeptidyl peptidase IV soluble form antibodyDipeptidylpeptidase 4 antibodyDipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibodyDipeptidylpeptidase IV antibodyDPP 4 antibodyDPP IV antibodyDPP IV estoenzyme antibodyDPP4 antibodyDPP4_HUMAN antibodyDPPIV antibodyIntestinal dipeptidyl peptidase antibodyT cell activation antigen CD26 antibodyT-cell activation antigen CD26 antibodyTP 103 antibodyTP103 antibody
      see all

    Anti-CD26 antibody [OX-61] (FITC) images

    • Flow Cytometry - CD26 antibody [OX-61] (FITC) (ab112227)
      Flow Cytometry - CD26 antibody [OX-61] (FITC) (ab112227)
      Cell Source: Thymus. ab112227 at a concentration of 0.5µg. Percentage of cells stained above control: 99.5 %.

    References for Anti-CD26 antibody [OX-61] (FITC) (ab112227)

    This product has been referenced in:
    • Cohen D  et al. Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells. Proc Natl Acad Sci U S A 101:13792-7 (2004). Read more (PubMed: 15365179) »
    • Sandhu JS  et al. Stem cell properties and repopulation of the rat liver by fetal liver epithelial progenitor cells. Am J Pathol 159:1323-34 (2001). Read more (PubMed: 11583960) »

    See all 3 Publications for this product

    Publishing research using ab112227 ? Please let us know so that we can cite the reference in this datasheet

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