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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Anti-CD26 antibody - Spacer region (ab28340)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28340? Please let us know so that we can cite the reference in this datasheet
ab28340 has been referenced in 1 publications.
- Pusch J et al. The physiological performance of a three-dimensional model that mimics the microenvironment of the small intestine. Biomaterials 32:7469-78 (2011). IHC-P; Human. PubMed: 21764120
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CD26 antibody - Spacer region (ab28340)
All lanes : Anti-CD26 antibody - Spacer region (ab28340) at 1/1000 dilution
Lane 1 : DPP-4 at 0.08 µg
Lane 2 : DPP-4 at 0.04 µg
Lane 3 : DPP-4 at 0.02 µg
Lane 4 : DPP-4 at 0.01 µg
Predicted band size : 88 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD26 antibody - Spacer region (ab28340)
ab28340 staining CD26 - Spacer region in rat epididymis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 and then blocking with 3% hydrogen peroxide in TBS for 10 minutes at 25°C was performed in order to block endogenous peroxidase activity. The primary antibody was used diluted in 1/400 and it was incubated with sample for 16 hours at 4°C in a dilution buffer containing TBS, 0.3% Triton X-100, 0.1 % BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.
This image is a courtesy of Veronique Dubois
CD26 antibody - Spacer region for IHC-P in Rat (28340)
CD26 antibody - Spacer region for WB in Human (28340)
CD26 antibody - Spacer region for WB in Cow (28340)
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