Products:Immunology >> Adaptive Immunity >> B Cells >> CD
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Dear Sir/Madam, We have troubles with the detection of the CD26 protein (antibody of COMPETITOR NAME) in our proximal tubule cell line, although we know that it should be present in these cells. On the Abcam website I found your antibody product ab28340, 100ug for 370euro. Because of the prize we would like to test a small amount of this antibody first, before we order this expensive antibody. Is it possible that we receive some ul’s of your antibody so we can perform a Westernblot on a few samples to test the specificity and detection properties of your antibody? With kind regards, |
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ANSWER: |
Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. This antibody is tested and guaranteed for WB and IHC-P in Mouse, Rat, and Human samples at this moment. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: http://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions. |
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I would like to know the homology for monkey. |
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ANSWER: |
Thank you for your enquiry. I have been in contact with the originator of these antibodies, and they are 99% homologous to chimp and so they should recognize your species. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CD26 antibody - Spacer region (ab28340) at 1/1000 dilution
Lane 1 : DPP-4 at 0.08 µg
Lane 2 : DPP-4 at 0.04 µg
Lane 3 : DPP-4 at 0.02 µg
Lane 4 : DPP-4 at 0.01 µg
Predicted band size : 88 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels.
ab28340 staining CD26 - Spacer region in rat epididymis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 and then blocking with 3% hydrogen peroxide in TBS for 10 minutes at 25°C was performed in order to block endogenous peroxidase activity. The primary antibody was used diluted in 1/400 and it was incubated with sample for 16 hours at 4°C in a dilution buffer containing TBS, 0.3% Triton X-100, 0.1 % BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.
This image is a courtesy of Veronique Dubois
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