![Flow Cytometry - CD274 antibody [MIH2] (Phycoerythrin) (ab80391) image](#CD274-Primary-antibodies-ab80391-1.gif)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD274 antibody [MIH2] (Phycoerythrin)
See all CD274 products (9) ...
Description
Mouse monoclonal [MIH2] to CD274 (Phycoerythrin)
Conjugation
Phycoerythrin
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Human CD274 transfected P815 cells
Positive control
Human peripheral blood monocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab80391 is prepared by affinity chromatography on Protein G from tissue culture supernatant.
Clonality
Monoclonal
Clone number
MIH2
Myeloma
P3U1
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry - CD274 antibody [MIH2] (Phycoerythrin) (ab80391)
![Flow Cytometry - CD274 antibody [MIH2] (Phycoerythrin) (ab80391) image](/ps/datasheet/images/80/ab80391/CD274-Primary-antibodies-ab80391-1.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
Flow Cyt
Application notes
Flow Cyt: Use 10µl neat for 106 cells in 100µl volume.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane and Endomembrane system.
Target information above from: UniProt accessionQ9NZQ7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B7 H1 antibody
- B7 homolog 1 antibody
- PD L1 antibody
see all
Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
Anti-CD274 antibody [MIH2] (Phycoerythrin) images:
Flow Cytometry - CD274 antibody [MIH2] (Phycoerythrin) (ab80391)
ab80391 at 10µl/106 cells staining CD274 in human peripheral blood monocytes by Flow cytometry.
References for Anti-CD274 antibody [MIH2] (Phycoerythrin) (ab80391)
ab80391 has not yet been referenced specifically in any publications.
Publishing research using ab80391? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"