![Flow Cytometry - CD274 antibody [MIH5] (Biotin) (ab95792) image](#CD274-Primary-antibodies-ab95792-2.jpg)
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Product name
Anti-CD274 antibody [MIH5] (Biotin)
See all CD274 products (9) ...
Description
Rat monoclonal [MIH5] to CD274 (Biotin)
Conjugation
Biotin
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
DBA/2 Mouse lymphoma L5178Y transfected with CD274 cDNA
Positive control
Mouse C57Bl/6 splenocytes
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, 150mM Sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
ab95792 is reported to be a blocking antibody.
Clonality
Monoclonal
Clone number
MIH5
Isotype
IgG2a
Light chain type
lambda
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry - CD274 antibody [MIH5] (Biotin) (ab95792)
![Flow Cytometry - CD274 antibody [MIH5] (Biotin) (ab95792) image](/ps/datasheet/images/95/ab95792/CD274-Primary-antibodies-ab95792-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab95792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.125-0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
Target
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane and Endomembrane system.
Target information above from: UniProt accessionQ9NZQ7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B7 H1 antibody
- B7 homolog 1 antibody
- PD L1 antibody
see all
Database links
- Entrez Gene: 60533 Mouse
- SwissProt: Q9EP73 Mouse
- Unigene: 245363 Mouse
Anti-CD274 antibody [MIH5] (Biotin) images:
Flow Cytometry - CD274 antibody [MIH5] (Biotin) (ab95792)
Flow cytometric staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 µg of ab95792 (filled histogram), followed by Streptavidin PE. Total viable cells were used for analysis.
References for Anti-CD274 antibody [MIH5] (Biotin) (ab95792)
This product has been referenced in:
- Sheng H et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res 18:846-57 (2008). Flow Cyt.Read more (PubMed: 18607390) »
- Saudemont A et al. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood 105:2428-35 (2005).Read more (PubMed: 15536145) »
See all 3 publications for this product
Publishing research using ab95792? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"