![Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276) image](#CD274-Primary-antibodies-ab80276-1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD274 antibody [MIH6] - Low Endotoxin
See all CD274 products (9) ...
Description
Rat monoclonal [MIH6] to CD274 - Low Endotoxin
Tested applications
Inhibition Assay, IP, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Mouse CD274-transfected L5178Y cells
Positive control
Peripheral mouse lymphocytes.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab80276 is prepared by affinity chromatography on Protein G from tissue culture supernatant.
Clonality
Monoclonal
Clone number
MIH6
Myeloma
P3U1
Isotype
IgG2a
Light chain type
lambda
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)
![Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276) image](/ps/datasheet/images/80/ab80276/CD274-Primary-antibodies-ab80276-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhib: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use 10µl for 106 cells.
Target
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane and Endomembrane system.
Target information above from: UniProt accessionQ9NZQ7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B7 H1 antibody
- B7 homolog 1 antibody
- PD L1 antibody
see all
Database links
- Entrez Gene: 60533 Mouse
- SwissProt: Q9EP73 Mouse
- Unigene: 245363 Mouse
Anti-CD274 antibody [MIH6] - Low Endotoxin images:
Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)
ab80276 at 10µl/106 cells staining CD274 in Peripheral Mouse Lymphocytes by Flow Cytometry.
References for Anti-CD274 antibody [MIH6] - Low Endotoxin (ab80276)
ab80276 has not yet been referenced specifically in any publications.
Publishing research using ab80276? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"