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Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)
![Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)](/ps/datasheet/images/80/ab80276/CD274-Primary-antibodies-ab80276-1.jpg)
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Product Name
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CD274 antibody [MIH6] - Low Endotoxin
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See all CD274 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [MIH6] to CD274 - Low Endotoxin
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Immunogen
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Mouse CD274-transfected L5178Y cells
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IP, Inhib
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 10µl for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Inhib: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Peripheral mouse lymphocytes.
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Cellular localization
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Type I membrane protein. Found on the plasma membrane (isoform 1) and in intracellular membrane (isoform 2).
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Research areas
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Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 60533 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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MIH6
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Myeloma
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P3U1
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Isotype
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IgG2a
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Light chain type
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lambda
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.4
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Purification notes
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ab80276 is prepared by affinity chromatography on Protein G from tissue culture supernatant.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD274 antibody [MIH6] - Low Endotoxin is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD274 antibody [MIH6] - Low Endotoxin images, references, products related to ab80276 and other tools.
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CD274 antibody [MIH6] - Low Endotoxin images:
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 Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)
ab80276 at 10µl/106 cells staining CD274 in Peripheral Mouse Lymphocytes by Flow Cytometry.
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Search PubMed (MEDLINE) for references to CD274
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CD274 antibody [MIH6] - Low Endotoxin - more information
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Customer reviews (feedback) regarding CD274 antibody [MIH6] - Low Endotoxin |
Customer FAQs regarding CD274 antibody [MIH6] - Low Endotoxin |
Protocols for CD274 antibody [MIH6] - Low Endotoxin |
Price and availability of products related to CD274 antibody [MIH6] - Low Endotoxin |
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CD274 antibody [MIH6] - Low Endotoxin - related products:
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CD274 antibody [MIH6] - Low Endotoxin - other tools:
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Contact Abcam with a Technical Enquiry about CD274 antibody [MIH6] - Low Endotoxin |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - CD274 antibody [MIH6] - Low Endotoxin (ab80276)](/index.html?datasheet=80276#CD274-Primary-antibodies-ab80276-1.jpg)
