Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD274 antibody
See all CD274 products (9) ...
Description
Goat polyclonal to CD274
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-K KQSDTHLEET, corresponding to C terminal amino acids 280-290 of Human CD274
C-K KQSDTHLEET
Positive control
Human heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
-
Western blot - CD274 antibody (ab28753)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
WB: Use a concentration of 0.3 - 2.0 µg/ml.Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).Can be blocked with CD274 peptide (280-290) (ab45676).
Target
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane and Endomembrane system.
Target information above from: UniProt accessionQ9NZQ7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B7 H1 antibody
- B7 homolog 1 antibody
- PD L1 antibody
see all
Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
Anti-CD274 antibody images:
Western blot - CD274 antibody (ab28753)
Anti-CD274 antibody (ab28753) at 0.3 µg/ml + human heart lysate 30µg total protein per lane
Predicted band size : 33 kDa
Observed band size : 37,39 kDa (why is the actual band size different from the predicted?)
References for Anti-CD274 antibody (ab28753)
ab28753 has not yet been referenced specifically in any publications.
Publishing research using ab28753? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"