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Western blot - CD274 antibody (ab28753)
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Product Name
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CD274 antibody
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See all CD274 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to CD274
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Immunogen
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Synthetic peptide: C-K KQSDTHLEET, corresponding to C terminal amino acids 280-290 of Human CD274
BLAST 'C-K KQSDTHLEET' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/32,000.
WB: Use at a concentration of 0.3 - 2.0 µg/ml. Detects bands of approximately 37 and 39 kDa (predicted molecular weight: 33 kDa). Can be blocked with CD274 peptide (280-290) (ab45676).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human heart lysate.
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Cellular localization
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Type I membrane protein. Found on the plasma membrane (isoform 1) and in intracellular membrane (isoform 2).
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Research areas
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Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD274 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD274 antibody images, references, products related to ab28753 and other tools.
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CD274 antibody images:
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Search PubMed (MEDLINE) for references to CD274
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CD274 antibody - more information
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Customer reviews (feedback) regarding CD274 antibody |
Customer FAQs regarding CD274 antibody |
Protocols for CD274 antibody |
Price and availability of products related to CD274 antibody |
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CD274 antibody - related products:
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CD274 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD274 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
Western blot - CD274 antibody (ab28753)