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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab58810? Please let us know so that we can cite the reference in this datasheet
ab58810 has been referenced in 1 publications.
- Cariani E et al. Immunological and molecular correlates of disease recurrence after liver resection for hepatocellular carcinoma. PLoS One 7:e32493 (2012). IHC-P; Human. PubMed: 22396772
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CD274 antibody (ab58810)
Lane 1 : Anti-CD274 antibody (ab58810) at 0.5 µg/ml
Lane 2 : Anti-CD274 antibody (ab58810) at 1 µg/ml
Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 33 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD274 antibody(ab58810)
IHC image of ab58810 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
CD274 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (58810)
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