If your product does not perform as described on this datasheet, we will refund or replace your product...
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab58810. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-CD274 antibody (ab58810) at 0.5 µg/ml
Lane 2 : Anti-CD274 antibody (ab58810) at 1 µg/ml
Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 33 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
IHC image of ab58810 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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