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Western blot - CD274 antibody (ab58810)
(enlarge)
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Product Name
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CD274 antibody
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See all CD274 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD274
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Immunogen
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17 amino acid peptide from near the centre of human CD274
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab58810 is specific to the larger isoform of CD274 (PDL1). It does not react with B7DC (PDL2).
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji cell lysate
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Cellular localization
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Type I membrane protein. Found on the plasma membrane (isoform 1) and in intracellular membrane (isoform 2).
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Research areas
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Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD274 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD274 antibody images, references, products related to ab58810 and other tools.
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CD274 antibody images:
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 Western blot - CD274 antibody (ab58810)
Lane 1 : CD274 antibody (ab58810) at 0.5 µg/ml
Lane 2 : CD274 antibody (ab58810) at 1 µg/ml
Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 33 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to CD274
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CD274 antibody - more information
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Customer reviews (feedback) regarding CD274 antibody |
Customer FAQs regarding CD274 antibody |
Protocols for CD274 antibody |
Price and availability of products related to CD274 antibody |
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CD274 antibody - related products:
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CD274 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD274 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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