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Anti-CD274 antibody (ab58810)

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Overview

Product name

Anti-CD274 antibody
See all CD274 products (9) ...

Description

Rabbit polyclonal to CD274

Specificity

ab58810 is specific to the larger isoform of CD274 (PDL1). It does not react with B7DC (PDL2).

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

17 amino acid peptide from near the centre of human CD274

Positive control

Raji cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Polyclonal

Isotype

IgG

  • Western blot - CD274 antibody (ab58810)Western blot - CD274 antibody (ab58810) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD274 antibody(ab58810)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD274 antibody(ab58810) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.5 - 1.0 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).

Target

Function

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Tissue specificity

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Cellular localization

Cell membrane and Endomembrane system.

Target information above from: UniProt accessionQ9NZQ7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B7 H1 antibody
  • B7 homolog 1 antibody
  • PD L1 antibody
  • PDCD1 ligand 1 antibody
  • PDCD1LG1 antibody
  • B7 H antibody
  • B7 homolog 1 antibody
  • B7-H1 antibody
  • B7H antibody
  • B7H1 antibody
  • CD 274 antibody
  • CD274 antibody
  • Cd274 antibody
  • CD274 antigen antibody
  • CD274 molecule antibody
  • MGC142294 antibody
  • MGC142296 antibody
  • OTTHUMP00000021029 antibody
  • PD-L1 antibody
  • PD1L1_HUMAN antibody
  • PDCD1 ligand 1 antibody
  • PDCD1L1 antibody
  • PDL 1 antibody
  • PDL1 antibody
  • Programmed cell death 1 ligand 1 antibody
  • Programmed death ligand 1 antibody
  • RGD1566211 antibody
see all

Anti-CD274 antibody images:

  Western blot - CD274 antibody (ab58810)

Western blot - CD274 antibody (ab58810)

Lane 1 : Anti-CD274 antibody (ab58810) at 0.5 µg/ml
Lane 2 : Anti-CD274 antibody (ab58810) at 1 µg/ml

Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 15 µg per lane.


Predicted band size : 33 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD274 antibody(ab58810)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD274 antibody(ab58810)

IHC image of ab58810 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD274 antibody (ab58810)

ab58810 has not yet been referenced specifically in any publications.

Publishing research using ab58810? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"