CD28 Human ELISA Kit (with color giving dyes) (ab119514)
Overview
- Product nameCD28 Human ELISA Kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall 5.9% Inter-assay Sample n Mean SD CV% Overall 7.2% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity0.18 ng/ml
- Range0.78 ng/ml - 50 ng/ml
- Recovery
117 %
Sample specific recovery Sample type Average % Range Serum 105 90% - 130% - Species reactivityReacts with: Human
- Product overview
ab119514 CD28 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human CD28. ab119514 is for research use only and is not for diagnostic or therapeutic procedures.
CD28 is a 44 kDa, disulphide-bonded, homodimeric glycoprotein, which is constitutively expressed on the surface of the majority of T-cells. CD28 is structurally very closely related to another molecule expressed in activated T-cells, CTLA-4. Ligation of CD28 by its counter receptor, B7, expressed on the surface of antigen presenting cells, has been shown to induce signals that, in synergy with those derived from engagement of the T-cell receptor by an antigen bound to a major histocompatibilty complex, enhance proliferation and cytokine production. Manipulation of this interaction can have dramatic effects on the outcome of T-cell activation. Considerable research has been done on the CD28/B7 costimulatory pathway. Blocking thereof results in immunosuppression with implications for the treatment of autoimmune diseases, allergy, organ transplantation and graft versus host disease.
Activating the CD28/B7 pathway could be useful for including the immune system to recognize and eliminate tumors that evade the immune system.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human sCD28 monoclonal antibody 1 x 100µl Blue-Dye 1 x 400µl Green-Dye 1 x 400µl Human sCD28 Standard 2 vials Monoclonal anti-human sCD28-coated microwell plates 1 unit Red-Dye 1 x 400µl Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml - Research Areas
Target information above from: UniProt accession
P10747
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD28CD28 antigenCD28 molecule
- CD28_HUMANT-cell-specific surface glycoprotein CD28TP44
see all
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
Applications
Our Abpromise guarantee covers the use of ab119514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
CD28 Human ELISA Kit (with color giving dyes) images
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Sandwich ELISA - CD28 Human ELISA Kit (with color giving dyes) (ab119514)Representative standard curve for ab119514.
Protocols
References for CD28 Human ELISA Kit (with color giving dyes) (ab119514)
ab119514 has not yet been referenced specifically in any publications.
