You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-CD28 antibody [CD28.2] (ab13463)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-CD28 antibody [CD28.2]
See all CD28 products (42) ...

Description

Mouse monoclonal [CD28.2] to CD28

Specificity

ab13463 recognizes the human CD28 antigen expressed by most T lineage cells. The antibody reacts with human CD28 transfected murine T cell hybridoma, PHA activated blasts and IL 6 dependent plasmacytoma lines. The antibody blocks CD28/B7 (CD80) mediated cell cell adhesion. It binds to the CDR domain in a CDR1 dependent way. Ab13463 may be used for: 1. Identification and enumeration of T lymphocytes in peripheral blood. 2. T cell isolation by sorting procedures. 3. Cell activation studies. 4. Cell adhesion studies.

Tested applications

Flow Cyt, ICC, IHC-Frmore details

Cross reactivity

Reacts with

Human, Rhesus monkey

Immunogen

Human CD28 transfected murine T cell hybridoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

CD28.2

Isotype

IgG1

Research areas

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     

  • ICC

     

  • IHC-Fr

     

Application notes

Flow Cyt: Use 5µl for 106 cells or 100 µl of whole blood.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Tissue specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionP10747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 28 antibody
  • CD28 antibody
  • CD28 antibody
  • CD28 antigen antibody
  • CD28 molecule antibody
  • CD28_HUMAN antibody
  • MGC138290 antibody
  • T cell antigen CD28 antibody
  • T cell specific surface glycoprotein antibody
  • T cell specific surface glycoprotein CD28 antibody
  • T-cell-specific surface glycoprotein CD28 antibody
  • Tp44 antibody
see all

Database links

References for Anti-CD28 antibody [CD28.2] (ab13463)

ab13463 has not yet been referenced specifically in any publications.

Publishing research using ab13463? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"