Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
Key features and details
- Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free
See all CD28 primary antibodies -
Description
Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to CD28. Human CD28 transfected murine T cell hybridoma.
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Positive control
- Flow Cyt: CD28 positive lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Azide free. -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Endotoxin level is less than or equal to 0.01 EU/ug, as determined by the LaL assay. -
Clonality
Monoclonal -
Clone number
CD28.2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Flow cytometry surface staining pattern of human peripheral blood stained using ab85986 GAM APC.
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Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab85986 GAM APC.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab85986 has been referenced in 11 publications.
- Liu R et al. Serum-derived exosomes containing NEAT1 promote the occurrence of rheumatoid arthritis through regulation of miR-144-3p/ROCK2 axis. Ther Adv Chronic Dis 12:2040622321991705 (2021). PubMed: 33995991
- Fehrholz M et al. Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. PLoS One 11:e0153578 (2016). PubMed: 27077658
- Urso K et al. NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis. Blood 118:795-803 (2011). PubMed: 21642596
- Chen ML et al. Antipsychotic drugs suppress the AKT/NF-?B pathway and regulate the differentiation of T-cell subsets. Immunol Lett 140:81-91 (2011). Functional Studies ; Human . PubMed: 21763349
- Scharschmidt E et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780