Anti-CD28 antibody (ab203084)
Key features and details
- Rabbit polyclonal to CD28
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-CD28 antibody
See all CD28 primary antibodies -
Description
Rabbit polyclonal to CD28 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide within Mouse CD28 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P31041 -
Positive control
- Mouse splenocytes; rat colitis and mouse lung tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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Flow Cyt |
1/20 - 1/100.
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Notes |
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IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Flow Cyt
1/20 - 1/100. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Entrez Gene: 12487 Mouse
- Entrez Gene: 25660 Rat
- Omim: 186760 Human
- SwissProt: P10747 Human
- SwissProt: P31041 Mouse
- SwissProt: P31042 Rat
- Unigene: 591629 Human
see all -
Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse lung tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Flow cytometric analysis of Mouse thymocytes labeling CD28 with ab203084 at 1/100 dilution for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab203084 has been referenced in 3 publications.
- Lu J et al. M2c Macrophages Protect Mice from Adriamycin-Induced Nephropathy by Upregulating CD62L in Tregs. Mediators Inflamm 2022:1153300 (2022). PubMed: 36262548
- Recasens M et al. Chronic exposure to IL-6 induces a desensitized phenotype of the microglia. J Neuroinflammation 18:31 (2021). PubMed: 33482848
- Xu Y et al. Immunohistochemical Biomarkers and Distribution of Telocytes in ApoE-/- Mice. Cell Biol Int N/A:N/A (2019). PubMed: 30912221