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Overview

  • Product nameCD299 protein (Fc Chimera)See all CD299 proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to extracellular domain amino acids 71-398 of Human CD299, fused to the Fc region of Human IgG1 (aa 93-330). The chimeric protein was expressed in modified Human 293 cells using a DNA sequence encoding the signal peptide of IL-10 receptor alpha chain fused to the CD299 (Fc chimera) sequence.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical sequence: HGTELPSPPSKLQVSKVPSSLSQEQSEQD AIYQNLTQLKAAVGELSEK SKLQEIYQELTQLKAAVGE LPEKSKLQEIYQELTRLKAAVGELPEKSK LQEIYQELT RLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQ EIYQELTELKAAVGELPEKSKLQEIYQELTQLKAAVGEL PDQSKQQQI YQELTDLKTAFERLCRHCPKDWTFFQGNC YFMSNSQRNWHDSVTACQE VRAQLVVIKTAEEQNFLQL QTSRSNRFSWMGLSDLNQEGTWQWVDGSP LSPSFQRYW NSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAA CFRDGIPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVF LFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYV DGVEVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGK EYKCRVSNKALPAPIEKTISKAKGQPREP QVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH NHYTQKSLS LSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83920 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    ab83920 migrates as a broad band between 70 and 85 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 65.6 kDa. ab83920 consists of 5-25% carbohydrate by weight.

    ab83920 separates into a number of glycoforms on 2D PAGE due to post-translational modifications, in particular glycosylation. The pI range is between 5.4 and 6.5. This compares with the unmodified CD299 (Fc Chimera) that has a predicted pI of 5.70.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C type lectin domain family 4, member MC-type lectin domain family 4 member MCD209 antigen like
        CD209 antigen like protein 1CD209 antigen-like protein 1CD209LCD209L1CD299CD299 antigenCLC4M_HUMANCLEC4MDC SIGN related proteinDC SIGN2DC SIGNRDC-SIGN-related proteinDC-SIGN2DC-SIGNRDCSIGN related proteinDCSIGNRDendritic cell-specific ICAM-3-grabbing non-integrin 2HP10347L SIGNL-SIGNLiver/lymph node specific ICAM3 grabbing nonintegrinLiver/lymph node-specific ICAM-3-grabbing non-integrinLSIGNMannose binding C type lectin DC SIGNRMGC129964MGC47866
      see all
  • FunctionProbable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
  • Tissue specificityPredominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q9H2X3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CD299 protein (Fc Chimera) images

    • SDS-PAGE - CD299 protein (Fc Chimera) (ab83920)
      SDS-PAGE - CD299 protein (Fc Chimera) (ab83920)
      Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 µg protein loaded per lane. Deep Purple stained.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein.
    • SDS-PAGE - CD299 protein (Fc Chimera) (ab83920)
      SDS-PAGE - CD299 protein (Fc Chimera) (ab83920)
      A sample of ab83920 without carrier protein was reduced and alkylated. 40 µg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as CD299 (Fc Chimera).
    • Functional Studies - CD299 protein (Fc Chimera) (ab83920)
      Functional Studies - CD299 protein (Fc Chimera) (ab83920)
      Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

      The triangle indicates theoretical pI and MW.

    References for CD299 protein (Fc Chimera) (ab83920)

    ab83920 has not yet been referenced specifically in any publications.

    Publishing research using ab83920 ? Please let us know so that we can cite the reference in this datasheet

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