CD299 protein (Fc Chimera) (ab83920)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab83920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab83920 migrates as a broad band between 70 and 85 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 65.6 kDa. ab83920 consists of 5-25% carbohydrate by weight.
ab83920 separates into a number of glycoforms on 2D PAGE due to post-translational modifications, in particular glycosylation. The pI range is between 5.4 and 6.5. This compares with the unmodified CD299 (Fc Chimera) that has a predicted pI of 5.70.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- C type lectin domain family 4, member MC-type lectin domain family 4 member MCD209 antigen like
- CD209 antigen like protein 1CD209 antigen-like protein 1CD209LCD209L1CD299CD299 antigenCLC4M_HUMANCLEC4MDC SIGN related proteinDC SIGN2DC SIGNRDC-SIGN-related proteinDC-SIGN2DC-SIGNRDCSIGN related proteinDCSIGNRDendritic cell-specific ICAM-3-grabbing non-integrin 2HP10347L SIGNL-SIGNLiver/lymph node specific ICAM3 grabbing nonintegrinLiver/lymph node-specific ICAM-3-grabbing non-integrinLSIGNMannose binding C type lectin DC SIGNRMGC129964MGC47866
CD299 protein (Fc Chimera) images
Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 µg protein loaded per lane. Deep Purple stained.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein.
A sample of ab83920 without carrier protein was reduced and alkylated. 40 µg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as CD299 (Fc Chimera).
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.The triangle indicates theoretical pI and MW.
References for CD299 protein (Fc Chimera) (ab83920)
ab83920 has not yet been referenced specifically in any publications.