Anti-CD2AP antibody (ab205017)
Key features and details
- Rabbit polyclonal to CD2AP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD2AP antibody -
Description
Rabbit polyclonal to CD2AP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD2AP. The exact sequence is proprietary. NP_036252.1.
Database link: Q9Y5K6 -
Positive control
- HeLa, Jurkat and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 71 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. -
Tissue specificity
Widely expressed in fetal and adult tissues. -
Involvement in disease
Defects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Contains 3 SH3 domains. -
Domain
The Pro-rich domain may mediate binding to SH3 domains.
Potential homodimerization is mediated by the coiled coil domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase. - Information by UniProt
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Database links
- Entrez Gene: 23607 Human
- Omim: 604241 Human
- SwissProt: Q9Y5K6 Human
- Unigene: 485518 Human
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Alternative names
- Adapter protein CMS antibody
- AL024079 antibody
- C78928 antibody
see all
Images
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All lanes : Anti-CD2AP antibody (ab205017) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 30 seconds -
Detection of CD2AP in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205017 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab205017 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab205017 has been referenced in 1 publication.
- Thuault S et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993