![Flow Cytometry - CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506) image](#CD3-Primary-antibodies-ab95506-1.jpg)
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Product name
Anti-CD3 antibody [145-2C11] (PE/Cy7 ®)
See all CD3 products (79) ...
Description
Armenian Hamster monoclonal [145-2C11] to CD3 (PE/Cy7 ®)
Conjugation
PE/Cy7 ®
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Mouse cytolytic T-cell clone
Positive control
C57BL/6 splenocytes; Mouse thymocyte suspensions.
General notes
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE/Cy7-conjugated antibodies, always protect samples from light.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: Less than or equal to 0.09% Sodium Azide
Constituents: PBS, pH 7.2 with BSA and/or Gelatin for protein stabilization
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
145-2C11
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
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Flow Cytometry - CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506)
![Flow Cytometry - CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506) image](/ps/datasheet/images/95/ab95506/CD3-Primary-antibodies-ab95506-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab95506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended.
Target
Function
The CD3 complex mediates signal transduction.
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similarities
Contains 1 ITAM domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP04234
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Database links
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12503 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12500 Mouse
- SwissProt: P22646 Mouse
- SwissProt: P24161 Mouse
- SwissProt: P11942 Mouse
- SwissProt: P04235 Mouse
see all
Anti-CD3 antibody [145-2C11] (PE/Cy7 ®) images:
Flow Cytometry - CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506)
Flow cytometry staining of C57BL/6 splenocytes with anti-CD45R (B220) PE and 0.5 µg of Armenian Hamster IgG Isotype Control PE/Cy7 (left) or 0.5 µg of ab95506 (right). Cells in the lymphocyte gate were used for analysis.
References for Anti-CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506)
This product has been referenced in:
- Geng H et al. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer 118:2657-64 (2006).Read more (PubMed: 16425224) »
- Krieg C et al. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol 175:6420-7 (2005).Read more (PubMed: 16272294) »
See all 4 publications for this product
Publishing research using ab95506? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"