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Anti-CD3 antibody [145-2C11] (ab91497)

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Overview

Product name

Anti-CD3 antibody [145-2C11]
See all CD3 products (79) ...

Description

Hamster monoclonal [145-2C11] to CD3

Specificity

ab91497 reacts with CD3 (epsilon subunit).

Tested applications

IP, IHC-Fr, Functional Studies, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Mouse BM10-37 cytotoxic T lymphocytes.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

Purified from cell culture supernatant by protein A affinity chromatography. Purity is > 95% (by SDS-PAGE).

Primary antibody notes

This antibody is commonly used as a phenotypic marker for Mouse T cells.

Clonality

Monoclonal

Clone number

145-2C11

Isotype

IgG

Research areas

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD

Applications

Show applications key

Our Abpromise guarantee covers the use of ab91497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IP

     

  • IHC-Fr

     

  • Functional Studies

     

  • ICC

     

  • Flow Cyt

     

Application notes

Flow Cyt: Use at a concentration of 1 µg/ml (106 cells).
FuncS: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at 1-2 µg / 100-500 µg protein in 1 ml cell lysate.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

The CD3 complex mediates signal transduction.

Involvement in disease

Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.

Sequence similarities

Contains 1 ITAM domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionP04234 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 4930549J05Rik antibody
  • A430104F18Rik antibody
  • AW552088 antibody
  • Cd247 antibody
  • CD247 antigen antibody
  • CD247 antigen, zeta subunit antibody
  • CD247 molecule antibody
  • CD3 antibody
  • CD3 delta antibody
  • CD3 epsilon antibody
  • CD3 eta antibody
  • CD3 gamma antibody
  • CD3 molecule delta polypeptide antibody
  • CD3 molecule, epsilon polypeptide antibody
  • CD3 molecule, gamma polypeptide antibody
  • CD3 zeta antibody
  • CD3D antibody
  • CD3D antigen delta polypeptide antibody
  • CD3d antigen, delta polypeptide (TiT3 complex) antibody
  • CD3d molecule delta antibody
  • CD3d molecule delta CD3 TCR complex antibody
  • CD3d molecule, delta (CD3-TCR complex) antibody
  • CD3D_HUMAN antibody
  • CD3E antibody
  • CD3e antigen antibody
  • CD3E antigen epsilon polypeptide antibody
  • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
  • CD3e molecule epsilon antibody
  • CD3e molecule epsilon CD3 TCR complex antibody
  • CD3e molecule, epsilon (CD3-TCR complex) antibody
  • CD3epsilon antibody
  • CD3G antibody
  • CD3g antigen antibody
  • CD3G antigen gamma polypeptide antibody
  • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
  • CD3g molecule gamma antibody
  • CD3g molecule gamma CD3 TCR complex antibody
  • CD3g molecule, gamma (CD3-TCR complex) antibody
  • CD3H antibody
  • CD3Q antibody
  • CD3Z antibody
  • CD3zeta antibody
  • Ctg3 antibody
  • FLJ17620 antibody
  • FLJ17664 antibody
  • FLJ18683 antibody
  • FLJ79544 antibody
  • FLJ94613 antibody
  • MGC138597 antibody
  • OKT3, delta chain antibody
  • OTTHUMP00000032544 antibody
  • T cell receptor antibody
  • T cell receptor T3 delta chain antibody
  • T cell receptor T3 gamma chain antibody
  • T cell receptor T3 zeta chain antibody
  • T cell receptor zeta chain antibody
  • T cell surface glycoprotein CD3 antibody
  • T cell surface glycoprotein CD3 delta chain antibody
  • T cell surface glycoprotein CD3 epsilon chain antibody
  • T cell surface glycoprotein CD3 gamma chain antibody
  • T cell surface glycoprotein CD3 zeta chain antibody
  • T-cell antigen receptor complex, epsilon subunit of T3 antibody
  • T-cell antigen receptor complex, gamma subunit of T3 antibody
  • T-cell antigen receptor complex, zeta subunit of CD3 antibody
  • T-cell receptor T3 delta chain antibody
  • T-cell receptor T3 gamma chain antibody
  • T-cell surface antigen T3/Leu-4 epsilon chain antibody
  • T-cell surface glycoprotein CD3 delta chain antibody
  • T-cell surface glycoprotein CD3 gamma chain antibody
  • T3d antibody
  • T3e antibody
  • T3g antibody
  • T3z antibody
  • TCRE antibody
  • TCRk antibody
  • Tcrz antibody
  • TCRzeta antibody
see all

Database links

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References for Anti-CD3 antibody [145-2C11] (ab91497)

This product has been referenced in:

  • Han WR  et al. Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts. Transpl Immunol 7:207-13 (1999).Read more (PubMed: 10638833) »
  • Kinnaert P  et al. Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies. Transpl Int 9:386-91 (1996).Read more (PubMed: 8819275) »

See all 6 publications for this product

Publishing research using ab91497? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"