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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD3 epsilon antibody [PPT3] (PE/Cy5®)
See all CD3 epsilon products (44) ...
Description
Mouse monoclonal [PPT3] to CD3 epsilon (PE/Cy5®)
Conjugation
PE/Cy5®
Tested applications
Cellular activation, Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Pig
Immunogen
The details of the immunogen for this antibody are not available.
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, 0.20% Gelatin, PBS and stabilizing agent.
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Porcine CD3e is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. ab25535 PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.
Clonality
Monoclonal
Clone number
PPT3
Isotype
IgG1
Light chain type
kappa
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cellular activation
Flow Cyt
IHC-Fr
IP
Application notes
In vitro activation of T cells: Use at an assay dependent dilution.
Flow Cyt: Use 0.2µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The CD3 complex mediates signal transduction.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP07766
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 3E antibody
- CD3 TCR complex antibody
- CD3E antibody
see all
Database links
References for Anti-CD3 epsilon antibody [PPT3] (PE/Cy5®) (ab25535)
This product has been referenced in:
- Yang Het al. Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes. Immunology 88:577-85 (1996).Read more (PubMed: 8881760) »
See 1 publication for this product
Publishing research using ab25535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"