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ab114153 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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looking for CD3 epsilon antibody, PE-conjugated, for dog and ICC/IF send info on conjugation kits send info about testing discount |
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ANSWER: |
Thank you for contacting us. Detailed information about our conjugation kits can be found here: www.abcam.com/easylink Please let me know if you have any specific questions in that regard or if I can help in selecting the right kit. For information about our testing discount program, please see below: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount. Please let me know which option would be best for you, and I'd be happy to send you either a discount code for testing a PE-conjugated CD3 epsilon antibody in dog and ICC/IF. If you decide to try ab26249 and conjugate it yourself, you would still be eligible for a testing discount code as ICC/IF has not been tested with this antibody. I hope this information is helpful to you. I look forward to hear back from you and assist you further. |
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What is the concentration of lot 177241? |
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ANSWER: |
Thank you for your enquiry. The concentration of the antibody is 100 ug/ml. I hope this information helps. Please do not hesitate to contact us again if you need anything further. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CD3 epsilon antibody (ab26249) at 1/500 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
ab26249 (2µg/ml) staining CD3 epsilon in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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