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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD3 epsilon antibody
See all CD3 epsilon products (44) ...
Description
Rabbit polyclonal to CD3 epsilon
Specificity
ab49943 reacts with the proline rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3 e chain. It may contain residual antibodies to thyroglobulin and may exhibit minimal background staining of epithelial and muscle tissues.
Tested applications
IHC-Fr, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Chicken, Cow, Cat, Dog, Human, Pig, Sand rat
Predicted to work with
Cynomolgus Monkey, Woodchuck
Immunogen
Synthetic human CD3 peptide (residues 156-168 of the CD3-e chain) coupled to porcine thyroglobulin.
KAKAKPVTRG AGA
Positive control
Extract of mouse thymus and human tonsil sections
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Purification notes
The antiserum has been treated to remove lipoproteins. Antibodies to thyroglobulin are largely depleted by solid phase immunoadsorption.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 18723761)
WB: 1/200Predicted molecular weight: 23 kDa.
IHC-P
IHC-P: 1/200IHC-P: 1/200
Target
Function
The CD3 complex mediates signal transduction.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP07766
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 3E antibody
- CD3 TCR complex antibody
- CD3E antibody
see all
Database links
- Entrez Gene: 396062 Chicken
- Entrez Gene: 281054 Cow
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 397455 Pig
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
- SwissProt: Q5R1Q1 Cat
see all
References for Anti-CD3 epsilon antibody (ab49943)
This product has been referenced in:
- Niida Het al. Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo. EMBO J 29:3558-70 (2010).Read more (PubMed: 20834228) »
- Olkhanud PBet al. Breast cancer lung metastasis requires expression of chemokine receptor CCR4 and regulatory T cells. Cancer Res 69:5996-6004 (2009). IHC-P; Mouse.Read more (PubMed: 19567680) »
See all 3 publications for this product
Publishing research using ab49943? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"