FITC Anti-CD3 zeta antibody [H146-968] (ab91493)
Key features and details
- FITC Armenian hamster monoclonal [H146-968] to CD3 zeta
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD3 zeta antibody [H146-968]
See all CD3 zeta primary antibodies -
Description
FITC Armenian hamster monoclonal [H146-968] to CD3 zeta -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Mouse CD3 zeta aa 150-250.
Database link: P20963 -
Positive control
- Flow Cyt: Murine splenocytes
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General notes
Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.00
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
H146-968 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Notes |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Target
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Function
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
Sequence similarities
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
Domain
The ITAM domains mediate interaction with SHB. -
Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281056 Cow
- Entrez Gene: 12503 Mouse
- Entrez Gene: 397302 Pig
- Entrez Gene: 100008617 Rabbit
- Entrez Gene: 25300 Rat
- SwissProt: P24161 Mouse
- SwissProt: Q9XSJ9 Pig
- SwissProt: Q9TUF8 Rabbit
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Intracellular staining flow cytometric pattern of murine splenocytes stained with ab91493: anti-cd3 zeta FITC antibody with a concentration of 3µg/ml in the sample
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Murine splenocytes mulitcolour intracellular staining flow cytometric pattern using 3µg/ml ab91493 and anti-human CD3 APC antibody with 10µl reagent per 100µl peripheral whole blood
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Flow cytometric intracellular staining analysis of murine splenocyte supension using anti-CD3 zeta FITC antibody (ab91493) sample concentation of of 3µg/ml. There is separation between the CD3 positive CD3 zeta positive splenocytes (red) and CD3 negative CD3 zeta negative splenocytes in black.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab91493 has been referenced in 2 publications.
- Pieren DKJ et al. Response kinetics reveal novel features of ageing in murine T cells. Sci Rep 9:5587 (2019). PubMed: 30944406
- Brazin KN et al. The T Cell Antigen Receptor α Transmembrane Domain Coordinates Triggering through Regulation of Bilayer Immersion and CD3 Subunit Associations. Immunity 49:829-841.e6 (2018). PubMed: 30389415