Overview

  • Product nameCD30 Mouse ELISA Kit
    See all CD30 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    12.29 pg/ml - 3000 pg/ml
  • Recovery

    76 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 77.29 69% - 87%
    Serum 75.99 68% - 84%
    Plasma 74.88 67% - 83%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s CD30 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD30 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD30 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD30 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD30 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD30 Mouse ELISA Kit images

  • Representative Standard Curve using ab113341

  • Representative Standard Curve using ab113341

Protocols

References for CD30 Mouse ELISA Kit (ab113341)

ab113341 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihren Anruf heute Morgen. Ich muss Ihnen leider bestätigen, dass die Klonnummer der in diesem Kit verwendeten Antikörper dem Betriebsgeheimnis unterliegt. Ich darf Ihnen aber weitergeben, dass der "Capture"- Antikörper monoklonal ist un...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"