CD31 Human ELISA Kit (ab100627)
- Product nameCD31 Human ELISA KitSee all CD31 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range8.23 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 113.8 105% - 121% Serum 106.1 95% - 114% Plasma 91.04 80% - 98%
- Species reactivityReacts with: Human
- Product overview
ab100627 Human CD31 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD31 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD31 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD31 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD31 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD31 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody PECAM-1 : biotinylated anti-human PECAM-1 2 vials PECAM 1 Microplate (12 strips x 8 wells) coated with anti-human PECAM-1 1 unit PECAM 1 Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
- CD31CD31 antigenEndoCAM
- GPIIAGPIIA''HEC7Leukocyte surface proteinPECA1PECA1_HUMANPECAM 1PECAM-1Pecam1Platelet endothelial cell adhesion moleculePlatelet/endothelial cell adhesion molecule
Our Abpromise guarantee covers the use of ab100627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD31 Human ELISA Kit (ab100627)
ab100627 has not yet been referenced specifically in any publications.