CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)
- Product nameCD31 Monkey ELISA Kit (with color giving dyes)See all CD31 kits ...
Intra-assay Sample n Mean SD CV% Overall 2%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity0.06 U/ml
- Range0.48 U/ml - 30 U/ml
- Species reactivityReacts with: Monkey
- Product overview
ab119552 CD31 Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for quantitative detection of soluble monkey CD31 (platelet endothelial cell adhesion molecule-1). ab119552 is for research use only and is not for use in diagnostic or therapeutic procedures.
CD31 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. CD31 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, CD31 was also detected on human, mouse and rat solid tumor lines.
Recent studies suggest a role for CD31 in the inflammatory process and leukocyte-endothelial interaction. CD31 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that CD31 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.
Besides the membrane-bound form of CD31 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated CD31, and contains the cytoplasmic tail. Soluble CD31 was detected in normal Human plasma.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 2 units Assay Buffer Concentrate (20x) 1 x 5ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml HRP-Conjugate anti-monkey-sCD31 monoclonal (murine) antibody 2 x 5µl Monkey sCD31 Standard 2 vials Monoclonal anti-monkey sCD31-coated microwell plate 1 unit Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
- CD31CD31 antigenEndoCAM
- GPIIAGPIIA''HEC7Leukocyte surface proteinPECA1PECA1_HUMANPECAM 1PECAM-1Pecam1Platelet endothelial cell adhesion moleculePlatelet/endothelial cell adhesion molecule
Our Abpromise guarantee covers the use of ab119552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)
ab119552 has not yet been referenced specifically in any publications.