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CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)

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Overview

  • Product nameCD31 Monkey ELISA Kit (with color giving dyes)See all CD31 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    0.06 U/ml
  • Range
    0.48 U/ml - 30 U/ml
  • Recovery

    110 %

  • Species reactivity
    Reacts with: Monkey
  • Product overview

    ab119552 CD31 Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for quantitative detection of soluble monkey CD31 (platelet endothelial cell adhesion molecule-1). ab119552 is for research use only and is not for use in diagnostic or therapeutic procedures.

    CD31 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. CD31 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, CD31 was also detected on human, mouse and rat solid tumor lines.

    Recent studies suggest a role for CD31 in the inflammatory process and leukocyte-endothelial interaction. CD31 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that CD31 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.

    Besides the membrane-bound form of CD31 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated CD31, and contains the cytoplasmic tail. Soluble CD31 was detected in normal Human plasma.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

    • Target information above from: UniProt accession P16284 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CD31CD31 antigenEndoCAM
        GPIIAGPIIA''HEC7Leukocyte surface proteinPECA1PECA1_HUMANPECAM 1PECAM-1Pecam1Platelet endothelial cell adhesion moleculePlatelet/endothelial cell adhesion molecule
      see all

    Applications

    Our Abpromise guarantee covers the use of ab119552 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA

    CD31 Monkey ELISA Kit (with color giving dyes) images

    • Sandwich ELISA - CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)
      Sandwich ELISA - CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)
      Representative standard curve using ab119552.

    Protocols

    References for CD31 Monkey ELISA Kit (with color giving dyes) (ab119552)

    ab119552 has not yet been referenced specifically in any publications.

    Publishing research using ab119552 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"