Recombinant Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6243] to CD32B + CD32A + CD32C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD32B + CD32A + CD32C antibody [EP6243]
See all CD32B+CD32A+CD32C primary antibodies -
Description
Rabbit monoclonal [EP6243] to CD32B + CD32A + CD32C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD32A aa 1-100 (extracellular). The exact sequence is proprietary.
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Positive control
- WB: Daudi and Raji cells. IHC-P: Human spleen and tonsil tissue. ICC/IF: Raji cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 13th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6243 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50.
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Notes |
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WB
1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50. |
Target
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Cellular localization
CD32B: Cell membrane. CD32A: Cell membrane. CD32C: Cytoplasm and Cell membrane. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Entrez Gene: 9103 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- SwissProt: P31995 Human
see all
Images
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All lanes : Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051) at 1/10000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34-37 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD32B + CD32A + CD32C with ab131051 at 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD32B + CD32A + CD32C with ab131051 at 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji cells labelling CD32B + CD32A + CD32C with ab131051 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab131051 overnight at 4° C. Nucleus were visualized using DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131051 has not yet been referenced specifically in any publications.