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ab17044 has been referenced in 10 publications.
Publishing research using ab17044? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-Interferon gamma antibody (ab10744) at 1/300 dilution
Lane 1 : whole cell lysate of hCD36-transfected HeLa cell line
Lane 2 : whole cell lysate of wild type HeLa cells
Lysates/proteins at 20 µg per lane.
Secondary
alkaline phosphatase conjugated goat anti-mouse antibody at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 85-88 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
The image was obtained using a colorimetric method of antigen detection, as a result of incubating the membrane with BCIP/NBP premixed solution (substrate for alkaline phosohatase) for 30 minutes at 4°C.
This image is courtesy of an anonymous Abreview
Anti-CD36 antibody [FA6-152] (ab17044) at 1/500 dilution + whole cell lysate prepared from human PBMCs at 20 µg
Secondary
IRDye 800CW conjugated donkey monoclonal at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 88 kDa (why is the actual band size different from the predicted?)
Blocking step performed using a special fluorescent blocking buffer at 50%, for 1 hour at room temperature.
Image courtesy of an anonymous Abreview.
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