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Western blot - CD36 antibody [FA6-152] (ab17044)
![Western blot - CD36 antibody [FA6-152] (ab17044)](/ps/datasheet/images/ab17044_1.jpg)
(enlarge)
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Product Name
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CD36 antibody [FA6-152]
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See all CD36 antibodies (21)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [FA6-152] to CD36
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Immunogen
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Fetal erythrocytes.
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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BL, Flow Cyt, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
BL: Use at an assay dependent dilution (blocks collagen/thrombospondin binding.
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 88 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Multi pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Atherosclerosis >> Lipid transport
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Microbiology >> Protein >> Human Protein >> Defensin
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets
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Relevance
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CD36 is a cell surface class B scavenger receptor. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cyto adherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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FA6-152
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 0.1% BSA, PBS.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD36 antibody [FA6-152] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD36 antibody [FA6-152] images, references, products related to ab17044 and other tools.
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CD36 antibody [FA6-152] images:
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 Western blot - CD36 antibody [FA6-152] (ab17044)
All lanes : Interferon gamma antibody (ab10744) at 1/300 dilution
Lane 1 : whole cell lysate of hCD36-transfected HeLa cell line
Lane 2 : whole cell lysate of wild type HeLa cells
Lysates/proteins at 20 µg per lane.
Secondary
alkaline phosphatase conjugated goat anti-mouse antibody at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 85-88 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
The image was obtained using a colorimetric method of antigen detection, as a result of incubating the membrane with BCIP/NBP premixed solution (substrate for alkaline phosohatase) for 30 minutes at 4°C. This image is courtesy of an anonymous Abreview
See Abreview
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References for CD36 antibody [FA6-152] (ab17044)
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This product has been used in: (two most recent references)
Reed-Geaghan EG et al. CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation. J Neurosci 29:11982-92 (2009). PubMed: 19776284
Houssier M et al. CD36 deficiency leads to choroidal involution via COX2 down-regulation in rodents. PLoS Med 5:e39 (2008). WB; Rat. PubMed: 18288886
See all 4 publication references for this product.
If you publish research using ab17044 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD36
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CD36 antibody [FA6-152] - more information
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Customer reviews (feedback) regarding CD36 antibody [FA6-152] |
Customer FAQs regarding CD36 antibody [FA6-152] |
Protocols for CD36 antibody [FA6-152] |
Price and availability of products related to CD36 antibody [FA6-152] |
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CD36 antibody [FA6-152] - related products:
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CD36 antibody [FA6-152] - other tools:
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Contact Abcam with a Technical Enquiry about CD36 antibody [FA6-152] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - CD36 antibody [FA6-152] (ab17044)](/index.html?datasheet=17044#ab17044_1.jpg)
