![Western blot - CD36 antibody [FA6-152] (ab17044) image](#ab17044_1.jpg)
![Western blot - CD36 antibody [FA6-152] (ab17044) image](#CD36-Primary-antibodies-ab17044-3.jpg)
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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-CD36 antibody [FA6-152]
See all CD36 products (22) ...
Description
Mouse monoclonal [FA6-152] to CD36
Tested applications
Blocking, Functional Studies, ICC/IF, WB, IHC-P, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
20-weeks-old fetal erythrocytes.
Positive control
HEL cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: None
Constituents: 0.1% BSA, PBS.
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
0.2 µm filtered
Clonality
Monoclonal
Clone number
FA6-152
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Atherosclerosis >> Lipid transport
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Microbiology >> Protein >> Human Protein >> Defensin
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
BL: Use at an assay dependent dilution. (Blocks collagen/thrombospondin binding.)
FuncS: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. (PubMed: 18288886)
IHC-P: Use at an assay dependent dilution. (PubMed: 17312010)
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
Involvement in disease
Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
Sequence similarities
Belongs to the CD36 family.
Post-translational
modifications
N-glycosylated and O-glycosylated with a ratio of 2:1.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adipocyte membrane protein antibody
- CD 36 antibody
- CD36 antibody
see all
Database links
- Entrez Gene: 948 Human
- Entrez Gene: 29184 Rat
- Omim: 173510 Human
- SwissProt: P16671 Human
- SwissProt: Q07969 Rat
- Unigene: 120949 Human
- Unigene: 102418 Rat
- Unigene: 205840 Rat
Anti-CD36 antibody [FA6-152] images:
Western blot - CD36 antibody [FA6-152] (ab17044)
![Western blot - CD36 antibody [FA6-152] (ab17044)](/ps/datasheet/Images/17/ab17044/ab17044_1.jpg)
All lanes : Anti-Interferon gamma antibody (ab10744) at 1/300 dilution
Lane 1 : whole cell lysate of hCD36-transfected HeLa cell line
Lane 2 : whole cell lysate of wild type HeLa cells
Lysates/proteins at 20 µg per lane.
Secondary
alkaline phosphatase conjugated goat anti-mouse antibody at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 85-88 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
The image was obtained using a colorimetric method of antigen detection, as a result of incubating the membrane with BCIP/NBP premixed solution (substrate for alkaline phosohatase) for 30 minutes at 4°C.
This image is courtesy of an anonymous Abreview
Western blot - CD36 antibody [FA6-152] (ab17044)
![Western blot - CD36 antibody [FA6-152] (ab17044)](/ps/datasheet/images/17/ab17044/CD36-Primary-antibodies-ab17044-3.jpg)
Anti-CD36 antibody [FA6-152] (ab17044) at 1/500 dilution + whole cell lysate prepared from human PBMCs at 20 µg
Secondary
IRDye 800CW conjugated donkey monoclonal at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 88 kDa (why is the actual band size different from the predicted?)
Blocking step performed using a special fluorescent blocking buffer at 50%, for 1 hour at room temperature.
Image courtesy of an anonymous Abreview.
References for Anti-CD36 antibody [FA6-152] (ab17044)
This product has been referenced in:
- Li Met al. High muscle lipid content in obesity is not due to enhanced activation of key triglyceride esterification enzymes or the suppression of lipolytic proteins. Am J Physiol Endocrinol Metab 300:E699-707 (2011). WB.Read more (PubMed: 21285405) »
- Díez Iet al. Nitric oxide, derived from inducible nitric oxide synthase, decreases hypoxia inducible factor-1alpha in macrophages during aspirin-induced mesenteric inflammation. Br J Pharmacol 159:1636-45 (2010). IHC-P; Rat.Read more (PubMed: 20233223) »
See all 9 publications for this product
Publishing research using ab17044? Please let us know so that we can cite the reference in this datasheet
![CD36 antibody [FA6-152] for Western blot in Human (17044)](/ps/datasheet/images/17/ab17044/CD36-Primary-antibodies-ab17044-2.jpg)
![CD36 antibody [FA6-152] for WB in Human (17044)](/ps/Reviews/Images/ab10718_55295.jpg)
![Anti-CD36 antibody [FA6-152] for Western blot in Human (17044)](/ps/datasheet/images/17/ab17044/CD36-Primary-antibodies-ab17044-7.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"