Anti-CD36 antibody [MF3] - Low Endotoxin (ab80080)

Overview

• Product nameAnti-CD36 antibody [MF3] - Low EndotoxinSee all CD36 primary antibodies ...
• Description
  Rat monoclonal [MF3] to CD36 - Low Endotoxin
• SpecificityAntibodies produced by clone MF3 have been shown to inhibit IL4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL4/GM-CSF-induced bone-marrorw derived macrophage fusion.
• Tested applicationsIHC-P, WB, IP, Functional Studies, Flow Cyt, ICC/IF, ICC more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  IL4 treated murine thioglycollate-elicited peritoneal macrophages.

• Positive controlMouse peripheral blood platelets.
• General notesEndotoxin Level <0.01EU/ug.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberMF3
• MyelomaY3/Ag1.2.3
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Stem Cells
  • Endothelial Progenitors
  • Endothelial Markers

  • Stem Cells
  • Hematopoietic Progenitors
  • Hematopoietic Stem Cells
  • Human Lineage Negative

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cardiovascular
  • Atherosclerosis
  • Lipid transport

  • Cardiovascular
  • Blood
  • Platelets

  • Microbiology
  • Protein
  • Human Protein
  • Defensin

  • Immunology
  • Cell Type Markers
  • CD
  • Platelets

Applications

Target

• FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
• Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
  Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
• Sequence similaritiesBelongs to the CD36 family.
• Post-translational
  modificationsN-glycosylated and O-glycosylated with a ratio of 2:1.
• Cellular localizationMembrane.

Target information above from: UniProt accession P16671 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 948 Human
  • Entrez Gene: 12491 Mouse
  • Omim: 173510 Human
  • SwissProt: P16671 Human
  • SwissProt: Q08857 Mouse
  • Unigene: 120949 Human
  • Unigene: 18628 Mouse
  • Unigene: 406799 Mouse

• Alternative names
  Adipocyte membrane protein antibodyCD 36 antibodyCD36 antibody

  CD36 antibodyCD36 antigen (collagen type I receptor, thrombospondin receptor) antibodyCD36 antigen antibodyCD36 molecule (thrombospondin receptor) antibodyCD36 molecule antibodyCD36_HUMAN antibodyCHDS7 antibodyCluster determinant 36 antibodyCollagen receptor, platelet antibodyFAT antibodyFatty acid translocase antibodyFatty acid transport protein antibodyGlycoprotein IIIb antibodyGP IIIb antibodygp3b antibodygp4 antibodygpiiib antibodygpiv antibodyLeukocyte differentiation antigen cd36 antibodyMGC108510 antibodyMGC91634 antibodyPAS 4 antibodyPAS 4 protein antibodyPAS IV antibodyPAS-4 antibodyPAS4 antibodypas4 protein antibodypasiv antibodyPlatelet collagen receptor antibodyPlatelet glycoprotein 4 antibodyPlatelet glycoprotein IV antibodyscarb3 antibodyScavenger receptor class B member 3 antibodyScavenger receptor class B, member 3 antibodyThrombospondin receptor antibodyThrombospondin receptor cluster determinant 36 antibody

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Anti-CD36 antibody [MF3] - Low Endotoxin images

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  Flow Cytometry - CD36 antibody [MF3] - Low Endotoxin (ab80080)
  ab80080 at 10µl/10⁶ cells staining CD36 in mouse peripheral blood platelets by Flow Cytometry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD36 antibody [MF3] - Low Endotoxin (ab80080)Image courtesy of Dr Jaykumar Thumar by Abreview.
  ab80080 staining CD36 in human coronary atherosclerosis plaques by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 0.1% BSA for 30 minutes at 36°C followed by incubation with the primary antibody at a 1/150 dilution for 24 hours at 4°C. A biotin conjugated rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

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• 

  Immunocytochemistry - Anti-CD36 antibody [MF3] - Low Endotoxin (ab80080)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa
  ab80080 staining CD36 - Low Endotoxin (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rat IgG polyclonal (1/750) was used as the secondary antibody.
  WGA (red) = Wheat Germ Agglutinin

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