Overview

  • Product nameAnti-CD36 antibody [VM58]
    See all CD36 primary antibodies
  • Description
    Mouse monoclonal [VM58] to CD36
  • SpecificityThe monoclonal antibody VM58 is directed against the CD36 antigen, a 90kD glycoprotein, also called GP IIIb expressed on human platelets. The GP IV (GP IIIb) molecule has been shown to function as thrombospondin receptor on platelets and monocytes and as receptor for Plasmodium falciparum infected erythrocytes. The monoclonal antibody reacts with platelets, monocytes, macrophages, erythroblasts and (weakly) with B-cells. In immunohistology the monoclonal antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin. The antibody VM58 induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human monocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab8145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ICC/IF
  • Application notesIF: Use at an assay dependent concentration. Fixation with 1% paraformaldehyde for 5 min. is advised.
    IHC-Fr: Use at an assay dependent dilution.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMultifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
      (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
    • Involvement in diseasePlatelet glycoprotein IV deficiency
      Coronary heart disease 7
    • Sequence similaritiesBelongs to the CD36 family.
    • Post-translational
      modifications
      N-glycosylated and O-glycosylated with a ratio of 2:1.
      Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
    • Cellular localizationCell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adipocyte membrane protein antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • Thrombospondin receptor antibody
      see all

    References for Anti-CD36 antibody [VM58] (ab8145)

    This product has been referenced in:
    • Vinogradov DV  et al. [Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex] Biokhimiia 56:787-97 (1991). Read more (PubMed: 1747408) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Below please find the link to a questionnaire that may help me in addition to what we discussed over the phone. Once you have submitted the form to us, we wil...

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    Thank you for your email. Ab8145 does cross-react with human and has been tested for application in IHC-frozen sections. You can read feedback from customers regarding this antibody by clicking on the reviews and enquiries tabs located on the online da...

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    Thank you for your enquiry. This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon g...

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    We curently do not stock, or are aware of any other company which produces, a commercially available control for this antibody. Since this antibody cross reacts with inflammatory cells and b-cells, maybe a section of lymphoid tissue such as spleen or t...

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    The antibody ab8145 to CD36 will work well with live cells. However, precautions should be taken to avoid aggregation of platelets that might be caused by this antibody.

    This antibody was raised in mouse and therefore it is unlikely that this antibody will cross-react with mouse or rat CD36

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"