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Overview

  • Product nameAnti-CD36 antibody [VM58]See all CD36 primary antibodies ...
  • Description
    Mouse monoclonal [VM58] to CD36
  • SpecificityThe monoclonal antibody VM58 is directed against the CD36 antigen, a 90kD glycoprotein, also called GP IIIb expressed on human platelets. The GP IV (GP IIIb) molecule has been shown to function as thrombospondin receptor on platelets and monocytes and as receptor for Plasmodium falciparum infected erythrocytes. The monoclonal antibody reacts with platelets, monocytes, macrophages, erythroblasts and (weakly) with B-cells. In immunohistology the monoclonal antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin. The antibody VM58 induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets.
  • Tested applicationsIHC-Fr, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human monocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab8145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr
ICC/IF
  • Application notesIF: Use at an assay dependent concentration. Fixation with 1% paraformaldehyde for 5 min. is advised.
    IHC-Fr: Use at an assay dependent dilution.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
    • Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
      Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
    • Sequence similaritiesBelongs to the CD36 family.
    • Post-translational
      modifications      N-glycosylated and O-glycosylated with a ratio of 2:1.
    • Cellular localizationMembrane.

      • Target information above from: UniProt accession P16671 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Adipocyte membrane protein antibodyCD 36 antibodyCD36 antibody
          CD36 antibodyCD36 antigen (collagen type I receptor, thrombospondin receptor) antibodycd36 antigen antibodyCD36 molecule (thrombospondin receptor) antibodyCD36 molecule antibodyCD36_HUMAN antibodyCHDS7 antibodyCluster determinant 36 antibodyCollagen receptor, platelet antibodyFAT antibodyfatty acid translocase antibodyFatty acid transport protein antibodyglycoprotein iiib antibodyGP IIIb antibodygp3b antibodygp4 antibodygpiiib antibodygpiv antibodyleukocyte differentiation antigen cd36 antibodyMGC108510 antibodyMGC91634 antibodyPAS 4 protein antibodyPAS IV antibodyPAS-4 antibodypas4 protein antibodypasiv antibodyplatelet collagen receptor antibodyPlatelet glycoprotein 4 antibodyplatelet glycoprotein iv antibodyscarb3 antibodyScavenger receptor class B member 3 antibodythrombospondin receptor antibody
        see all

      References for Anti-CD36 antibody [VM58] (ab8145)

      This product has been referenced in:
      • Vinogradov DV  et al. [Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex] Biokhimiia 56:787-97 (1991). Read more (PubMed: 1747408) »

      See 1 Publication for this product

      Publishing research using ab8145 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab8145 Submit a Question for ab8145

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First

      The ds when we received the antibody said tested in IHC and also tested in IHC-Fr. We assumed this meant it was tested in IHC-P and IHC-Fr. We use the Ventana system. We have tried the following HIER: citrate pH 6, EDTA pH 9 and PIER: protease 2. We u...

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      Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Below please find the link to a questionnaire that may help me in addition to what we discussed over the phone. Once you have submitted the form to us, we will l...

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      PRE-SALES ENQUIRY I'm very interested in this antibody. Can I use it for ABC IHC with human frozen paraformaldehyde fixed tissues ?

      Thank you for your email. Ab8145 does cross-react with human and has been tested for application in IHC-frozen sections. You can read feedback from customers regarding this antibody by clicking on the reviews and enquiries tabs located on the online dat...

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      Could your product of"CD36 antibody (ab8145)" be applied to immunohistochemistry on paraffin-embeded tissue sections? Thank you.

      Thank you for your enquiry. This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift...

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      Do you have any sort of positive control for this antibody commercially available? If you know of any other compnaies that have one please let me know.

      We curently do not stock, or are aware of any other company which produces, a commercially available control for this antibody. Since this antibody cross reacts with inflammatory cells and b-cells, maybe a section of lymphoid tissue such as spleen or to...

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      I read that for fluorescence technique on cells fixation it is advised. I cannot fix cells before the staining for the analysis by cytolfuorometry is the antibody working well on live cells.

      The antibody ab8145 to CD36 will work well with live cells. However, precautions should be taken to avoid aggregation of platelets that might be caused by this antibody.

      Does the ab recognize CD36 in rat or mouse tissues?

      This antibody was raised in mouse and therefore it is unlikely that this antibody will cross-react with mouse or rat CD36

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"