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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-CD36 antibody [VM58]
See all CD36 products (22) ...
Description
Mouse monoclonal [VM58] to CD36
Specificity
The monoclonal antibody VM58 is directed against the CD36 antigen, a 90kD glycoprotein, also called GP IIIb expressed on human platelets. The GP IV (GP IIIb) molecule has been shown to function as thrombospondin receptor on platelets and monocytes and as receptor for Plasmodium falciparum infected erythrocytes. The monoclonal antibody reacts with platelets, monocytes, macrophages, erythroblasts and (weakly) with B-cells. In immunohistology the monoclonal antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin. The antibody VM58 induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets.
Tested applications
IHC-Fr, IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Human monocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS & Sodium Azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Ammonium sulfate + DEAE-cellulose, gradient elution
Clonality
Monoclonal
Clone number
VM58
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Atherosclerosis >> Lipid transport
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Microbiology >> Protein >> Human Protein >> Defensin
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
Application notes
IF: Use at an assay dependent concentration. Fixation with 1% paraformaldehyde for 5 min. is advised.
IHC-Fr: Use at an assay dependent dilution.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
Involvement in disease
Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
Sequence similarities
Belongs to the CD36 family.
Post-translational
modifications
N-glycosylated and O-glycosylated with a ratio of 2:1.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adipocyte membrane protein antibody
- CD 36 antibody
- CD36 antibody
see all
Database links
- Entrez Gene: 948 Human
- Omim: 173510 Human
- SwissProt: P16671 Human
- Unigene: 120949 Human
References for Anti-CD36 antibody [VM58] (ab8145)
This product has been referenced in:
- Vinogradov DVet al. [Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex] Biokhimiia 56:787-97 (1991).Read more (PubMed: 1747408) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"