Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab78054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.

Seeing WB bands at 75 and/or 84 kDa is common for CD36 due to differential glycosylation

ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
  • Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
    Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
  • Sequence similaritiesBelongs to the CD36 family.
  • Post-translational
    modifications
    N-glycosylated and O-glycosylated with a ratio of 2:1.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte membrane protein antibody
    • CD36 antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • GP3B antibody
    • GP4 antibody
    • GPIIIB antibody
    • GPIV antibody
    • Leukocyte differentiation antigen CD36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PASIV antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Thrombospondin receptor antibody
    see all

Anti-CD36 antibody images

  • All lanes : Anti-CD36 antibody (ab78054)

    Lane 1 : Lysate prepared from human platelets at 15 µg
    Lane 2 : Lysate prepared from human platelets at 30 µg


    Predicted band size : 53 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, staining CD36 with ab78054 at 5 µg/ml.
    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue, staining CD36 with ab78054 at 5 µg/ml.
    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, staining CD36 with ab78054 at 5 µg/ml.
    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, staining CD36 with ab78054 at 5 µg/ml.
    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-CD36 antibody (ab78054)

This product has been referenced in:
  • Jové M  et al. Lipidomic and metabolomic analyses reveal potential plasma biomarkers of early atheromatous plaque formation in hamsters. Cardiovasc Res 97:642-52 (2013). Read more (PubMed: 23241314) »
  • Smith DD  et al. Mast cell deficiency attenuates progression of atherosclerosis and hepatic steatosis in apolipoprotein E-null mice. Am J Physiol Heart Circ Physiol 302:H2612-21 (2012). IHC-P ; Mouse . Read more (PubMed: 22505639) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Follicle)
Specification Follicle
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 18 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 25 2014

Abreviews
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Treatment Day 0 and day 7 post-MI
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Merry Lindsey

Verified customer

Submitted Sep 20 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: antigen retrieval
Sample Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
Specification Heart at day 0 and day 7 post-MI
Permeabilization No
Fixative 10% zinc formalin
Username

Dr. Merry Lindsey

Verified customer

Submitted Sep 20 2013

Application Flow Cytometry
Fixation None
Permeabilization No
Sample Human Cell (HSC)
Specification HSC
Gating Strategy No gate
Preparation Cell harvesting/tissue preparation method: Fresh Whole Blood
Sample buffer: PBS
Username

Abcam user community

Verified customer

Submitted Aug 20 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for sending the details of your protocol and the blot image. Are you confident that the secondary antibody is effective? I think your protocol is good, and that your samples are good too, assuming these are the same as the samples that you b...

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Thank you for contacting us. Ab78054 is indeed a very good option for detecting CD36 in formalin-fixed paraffin-embedded (FFPE) human tissue. There are other alternatives in our catalogue, depending on the clonality and species it reacts with, you ca...

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Application Immunocytochemistry
Sample Human Cell (Human Platelets)
Specification Human Platelets
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"