Overview

  • Product nameAnti-CD36 antibody
    See all CD36 primary antibodies
  • Description
    Rabbit polyclonal to CD36
  • Tested applicationsICC, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide:

    AKENVTQDAEDNTVSF

    , corresponding to amino acids 99-114 of Human CD36

  • Positive control
    • Heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab78054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
    • Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
      Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
    • Sequence similaritiesBelongs to the CD36 family.
    • Post-translational
      modifications
      N-glycosylated and O-glycosylated with a ratio of 2:1.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adipocyte membrane protein antibody
      • CD36 antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • Thrombospondin receptor antibody
      see all

    Anti-CD36 antibody images

    • Immunohistochemical staining of paraffin embedded, formalin fixed, human splenic endothelium using ab78054 at a dilution of 1/200.

    • Negative control - immunohistochemical staining of paraffin-embedded, formalin fixed, thymic lymphocytes using ab78054 at a dilution of 1/200.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    References for Anti-CD36 antibody (ab78054)

    This product has been referenced in:
    • Jové M  et al. Lipidomic and metabolomic analyses reveal potential plasma biomarkers of early atheromatous plaque formation in hamsters. Cardiovasc Res 97:642-52 (2013). Read more (PubMed: 23241314) »
    • Smith DD  et al. Mast cell deficiency attenuates progression of atherosclerosis and hepatic steatosis in apolipoprotein E-null mice. Am J Physiol Heart Circ Physiol 302:H2612-21 (2012). IHC-P ; Mouse . Read more (PubMed: 22505639) »

    See all 6 Publications for this product

    Product Wall

    Abreviews
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Sample Mouse Tissue lysate - whole (Liver)
    Specification Liver
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (Follicle)
    Specification Follicle
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 18 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (tooth)
    Specification tooth
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Abreviews
    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
    Sample Mouse Tissue lysate - whole (Heart)
    Specification Heart
    Treatment Day 0 and day 7 post-MI
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Merry Lindsey

    Verified customer

    Submitted Sep 20 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: antigen retrieval
    Sample Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
    Specification Heart at day 0 and day 7 post-MI
    Permeabilization No
    Fixative 10% zinc formalin
    Username

    Dr. Merry Lindsey

    Verified customer

    Submitted Sep 20 2013

    Application Flow Cytometry
    Fixation None
    Permeabilization No
    Sample Human Cell (HSC)
    Specification HSC
    Gating Strategy No gate
    Preparation Cell harvesting/tissue preparation method: Fresh Whole Blood
    Sample buffer: PBS
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

    Read More

    Thank you for sending the details of your protocol and the blot image. Are you confident that the secondary antibody is effective? I think your protocol is good, and that your samples are good too, assuming these are the same as the samples that you b...

    Read More

    Thank you for contacting us. Ab78054 is indeed a very good option for detecting CD36 in formalin-fixed paraffin-embedded (FFPE) human tissue. There are other alternatives in our catalogue, depending on the clonality and species it reacts with, you ca...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (Human Platelets)
    Specification Human Platelets
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Dec 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"