Overview

  • Product nameAnti-CD36 antibody
    See all CD36 primary antibodies
  • Description
    Rabbit polyclonal to CD36
  • Tested applicationsSuitable for: ICC, ELISA, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide:

    AKENVTQDAEDNTVSF

    , corresponding to amino acids 99-114 of Human CD36

  • Positive control
    • Heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab78054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionMultifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
      (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
    • Involvement in diseasePlatelet glycoprotein IV deficiency
      Coronary heart disease 7
    • Sequence similaritiesBelongs to the CD36 family.
    • Post-translational
      modifications
      N-glycosylated and O-glycosylated with a ratio of 2:1.
      Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
    • Cellular localizationCell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adipocyte membrane protein antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • Thrombospondin receptor antibody
      see all

    Anti-CD36 antibody images

    • Immunohistochemical staining of paraffin embedded, formalin fixed, human splenic endothelium using ab78054 at a dilution of 1/200.

    • Negative control - immunohistochemical staining of paraffin-embedded, formalin fixed, thymic lymphocytes using ab78054 at a dilution of 1/200.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, staining CD36 with ab78054 at 5 µg/ml.
      A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    References for Anti-CD36 antibody (ab78054)

    This product has been referenced in:
    • Bhat OM  et al. Interleukin-18-induced atherosclerosis involves CD36 and NF-?B crosstalk in Apo E-/- mice. J Cardiol 66:28-35 (2015). Read more (PubMed: 25475966) »
    • Jové M  et al. Lipidomic and metabolomic analyses reveal potential plasma biomarkers of early atheromatous plaque formation in hamsters. Cardiovasc Res 97:642-52 (2013). Read more (PubMed: 23241314) »

    See all 7 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rabbit Tissue sections (Insitional back wound)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization No
    Specification Insitional back wound
    Blocking step Dako Dual Endogenous Enzyme Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 29 2016

    Abreviews
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Sample Mouse Tissue lysate - whole (Liver)
    Specification Liver
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (Follicle)
    Specification Follicle
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 18 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (tooth)
    Specification tooth
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Abreviews
    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
    Sample Mouse Tissue lysate - whole (Heart)
    Specification Heart
    Treatment Day 0 and day 7 post-MI
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Merry Lindsey

    Verified customer

    Submitted Sep 20 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: antigen retrieval
    Sample Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
    Specification Heart at day 0 and day 7 post-MI
    Permeabilization No
    Fixative 10% zinc formalin
    Username

    Dr. Merry Lindsey

    Verified customer

    Submitted Sep 20 2013

    Application Flow Cytometry
    Fixation None
    Permeabilization No
    Sample Human Cell (HSC)
    Specification HSC
    Gating Strategy No gate
    Preparation Cell harvesting/tissue preparation method: Fresh Whole Blood
    Sample buffer: PBS
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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    Thank you for sending the details of your protocol and the blot image. Are you confident that the secondary antibody is effective? I think your protocol is good, and that your samples are good too, assuming these are the same as the samples that you b...

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    Thank you for contacting us. Ab78054 is indeed a very good option for detecting CD36 in formalin-fixed paraffin-embedded (FFPE) human tissue. There are other alternatives in our catalogue, depending on the clonality and species it reacts with, you ca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"