Overview
- Product nameAnti-CD36 antibodySee all CD36 primary antibodies ...
- DescriptionRabbit polyclonal to CD36
- Tested applicationsICC, WB, ELISA, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit - Immunogen
Synthetic peptide: AKENVTQDAEDNTVSF, corresponding to amino acids 99-114 of Human CD36
- Positive controlHeart tissue
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline, 50% Glycerol -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab78054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent concentration. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 53 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
- Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938]. - Sequence similaritiesBelongs to the CD36 family.
- Post-translational
modificationsN-glycosylated and O-glycosylated with a ratio of 2:1. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 948 Human
- Entrez Gene: 12491 Mouse
- Entrez Gene: 29184 Rat
- Omim: 173510 Human
- SwissProt: P16671 Human
- SwissProt: Q08857 Mouse
- SwissProt: Q07969 Rat
- Unigene: 120949 Human
- Unigene: 18628 Mouse
- Unigene: 406799 Mouse
- Unigene: 102418 Rat
- Unigene: 205840 Rat
see all
Target information above from: UniProt accession
P16671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Adipocyte membrane protein antibodyCD36 antibodyCD36 antibody
- CD36 antigen (collagen type I receptor, thrombospondin receptor) antibodyCD36 antigen antibodyCD36 molecule (thrombospondin receptor) antibodyCD36 molecule antibodyCD36_HUMAN antibodyCHDS7 antibodyCluster determinant 36 antibodyCollagen receptor, platelet antibodyFAT antibodyFatty acid translocase antibodyFatty acid transport protein antibodyGlycoprotein IIIb antibodyGP IIIb antibodyGP3B antibodyGP4 antibodyGPIIIB antibodyGPIV antibodyLeukocyte differentiation antigen CD36 antibodyMGC108510 antibodyMGC91634 antibodyPAS 4 protein antibodyPAS IV antibodyPAS-4 antibodyPASIV antibodyPlatelet collagen receptor antibodyPlatelet glycoprotein 4 antibodyPlatelet glycoprotein IV antibodyscarb3 antibodyScavenger receptor class B member 3 antibodyThrombospondin receptor antibody
see all
Anti-CD36 antibody images
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Western blot - CD36 antibody (ab78054)All lanes : Anti-CD36 antibody (ab78054)
Lane 1 : Lysate prepared from human platelets at 15 µg
Lane 2 : Lysate prepared from human platelets at 30 µg
Predicted band size : 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD36 antibody (ab78054)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, staining CD36 with ab78054 at 5 µg/ml. A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD36 antibody (ab78054)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue, staining CD36 with ab78054 at 5 µg/ml. A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD36 antibody (ab78054)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, staining CD36 with ab78054 at 5 µg/ml. A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody (ab78054)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, staining CD36 with ab78054 at 5 µg/ml. A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-CD36 antibody (ab78054)
This product has been referenced in:
- Jové M et al. Lipidomic and metabolomic analyses reveal potential plasma biomarkers of early atheromatous plaque formation in hamsters. Cardiovasc Res 97:642-52 (2013). Read more (PubMed: 23241314) »
- Smith DD et al. Mast cell deficiency attenuates progression of atherosclerosis and hepatic steatosis in apolipoprotein E-null mice. Am J Physiol Heart Circ Physiol 302:H2612-21 (2012). IHC-P ; Mouse . Read more (PubMed: 22505639) »
