CD38 antibody [HIT2] (ab2577)

Product Name 

CD38 antibody [HIT2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [HIT2] to CD38

Immunogen 

Tissue / cell preparation (Human thymocytes in foetus).

Reacts with 
(species key)

Hu

Specificity 

CD38 antigen

Tested applications 
(see key)

Flow Cyt, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
WB: Use at an assay dependant concentration. Use under non-reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL).

Cellular localization 

Membrane; single pass type II membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD38 (T10) is a single chain 42 to 46 kDa type II integral transmembrane glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. It is expressed by murine, but not human, hematopoietic progenitors. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.

In mice, CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and is not expressed by mature plasma cells. Murine CD38 is also expressed by all Mac1⁺ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone marrow derived macrophages. This pattern of expression contrasts strikingly with that seen in the human.

Monoclonal antibodies to CD38 have been shown to be useful in subtyping of lymphomas and leukemias, inhibition of B lymphopoiesis, detection of plasma cells, protection of B cells from apoptosis, and as a marker for activated B and T cell proliferation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

HIT2

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites by protein G-affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CD38 antibody [HIT2] (ab2577)

  This product has been used in: (two most recent references)

Joshua D et al. The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis. Br J Haematol 94:76-81 (1996). PubMed: 8757512

Schlossman et al. (Eds.) Leucocyte typing V. Oxford University Press : (1995).

See all 4 publication references for this product.


If you publish research using ab2577 please let us know so that we can cite the reference on this datasheet.

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