CD38 antibody [NIMR-5] (FITC) (ab25667)

Product Name 

CD38 antibody [NIMR-5] (FITC)

Product type 

Primary antibodies

Description 

Rat monoclonal [NIMR-5] to CD38 (FITC)

Immunogen 

The details of the immunogen for this antibody are not available.

Reacts with 
(species key)

Ms

Specificity 

ab25667 recognises murine CD38.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 1µg for 10⁶ cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Induction of B lymphocyte proliferation: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single pass type II membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD38 (T10) is a single chain 42 to 46 kDa type II integral transmembrane glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. It is expressed by murine, but not human, hematopoietic progenitors. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.

In mice, CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and is not expressed by mature plasma cells. Murine CD38 is also expressed by all Mac1⁺ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone marrow derived macrophages. This pattern of expression contrasts strikingly with that seen in the human.

Monoclonal antibodies to CD38 have been shown to be useful in subtyping of lymphomas and leukemias, inhibition of B lymphopoiesis, detection of plasma cells, protection of B cells from apoptosis, and as a marker for activated B and T cell proliferation.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

NIMR-5

Isotype 

IgG2a

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C in the dark.

Customer reviews (feedback) regarding CD38 antibody [NIMR-5] (FITC)

Customer FAQs regarding CD38 antibody [NIMR-5] (FITC)

Protocols for CD38 antibody [NIMR-5] (FITC)

Price and availability of products related to CD38 antibody [NIMR-5] (FITC)

CD38 antibody [NIMR-5] (FITC) - related products: