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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab24894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
Flow Cytometry analysis of Feline peripheral blood lymphocytes labeling CD4 with ab24894 at 1 μg/106 cells. A Mouse Anti-Feline CD8-PE was used as the secondary antibody.
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 (at 1 µg/106 cells) and a PE conjugated mouse anti-feline CD8 antibody (ab25417). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 and a PE conjugated mouse anti-feline CD5 antibody (ab25415, at 0.2 µg/106 cells). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).
ab24894 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"