Post-translational modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 (at 1 µg/106 cells) and a PE conjugated mouse anti-feline CD8 antibody (ab25417). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 and a PE conjugated mouse anti-feline CD5 antibody (ab25415, at 0.2 µg/106 cells). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).
References for Anti-CD4 antibody [3-4F4] (FITC) (ab24894)
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