Post-translational modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Peripheral blood mononuclear cells were isolated from
heparinized pig blood on Ficoll-Hypaque density gradients and double-stained with ab25354 at 0.2ug/106 cells and mouse anti-pig CD8a-FITC. Lymphocytes were gated andanalyzed on a FACScan• flow cytometer (BDIS, San Jose, CA).
References for Anti-CD4 antibody [74-12-4] (Phycoerythrin) (ab25354)
has not yet been referenced specifically in any publications.
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