Anti-CD4 antibody [B-A1] (FITC) (ab59474)

Overview

  • Product nameAnti-CD4 antibody [B-A1] (FITC)See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [B-A1] to CD4 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityRecognises a 55 kDa protein
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey

    Does not react with

    Mouse
  • Immunogen

    PHA activated T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab59474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl for 106 cells or 100µl of blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Target information above from: UniProt accession P01730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [B-A1] (FITC) images

    • A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.
    • Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

      Gating Strategy: Live lymphocytes

      See Abreview

    References for Anti-CD4 antibody [B-A1] (FITC) (ab59474)

    ab59474 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Permeabilization No
    Sample Cynomolgus Monkey Cell (blood)
    Specification blood
    Gating Strategy alive lymphocytes
    Preparation Cell harvesting/tissue preparation method: red blood cell lysis
    Sample buffer: PBS/BSA 0.5%
    Username

    Abcam user community

    Verified customer

    Submitted Dec 24 2013

    Application Flow Cytometry
    Permeabilization No
    Sample Human Cell (PBMC)
    Specification PBMC
    Gating Strategy alive lymphocytes
    Preparation Cell harvesting/tissue preparation method: ficoll
    Sample buffer: PBS/BSA 0.5%
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Application Flow Cytometry
    Sample Rat Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 10 2013



    500 uL of the antibody is provided per 50-test vial.

    Application Flow Cytometry
    Sample Human Cell (from buffy coat)
    Specification from buffy coat
    Preparation Cell harvesting/tissue preparation method: after ficoll extraction
    Sample buffer: PBS/BSA 1%
    Fixation none
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 04 2013

    Application Flow Cytometry
    Sample Mouse Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Application Flow Cytometry
    Sample Cynomolgus Monkey Cell (from whole blood)
    Specification from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Thank you for contacting Abcam.
    All of the antibodies that you mentioned and all the antibodies in our catalogue are guaranteed for 6 months under our Abpromise. In terms of stability, they should all have be the same, regardless of the BSA concen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"