Overview

  • Product name
    Anti-CD4 antibody [B-A1] (FITC)
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [B-A1] to CD4 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Recognises a 55 kDa protein
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    PHA activated T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab59474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells or 100µl of blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similarities
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localization
      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
      • Alternative names
        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Images

      • A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.
      • Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

        Gating Strategy: Live lymphocytes

        See Abreview

      References

      This product has been referenced in:
      • Sui M  et al. Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer. Oncol Lett 12:4087-4093 (2016). Flow Cyt ; Human . Read more (PubMed: 27895776) »

      See 1 Publication for this product

      Customer reviews and Q&As

      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Flow Cytometry
      Sample
      Rat Cell (Splenocytes)
      Specification
      Splenocytes
      Preparation
      Cell harvesting/tissue preparation method: isolated from spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Username

      Abcam user community

      Verified customer

      Submitted Jan 10 2013



      500 uL of the antibody is provided per 50-test vial.

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Flow Cytometry
      Sample
      Human Cell (from buffy coat)
      Specification
      from buffy coat
      Preparation
      Cell harvesting/tissue preparation method: after ficoll extraction
      Sample buffer: PBS/BSA 1%
      Fixation
      none
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Username

      Abcam user community

      Verified customer

      Submitted Jan 04 2013

      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Flow Cytometry
      Sample
      Mouse Cell (Splenocytes)
      Specification
      Splenocytes
      Preparation
      Cell harvesting/tissue preparation method: isolated from spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Username

      Abcam user community

      Verified customer

      Submitted Jan 02 2013

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Flow Cytometry
      Sample
      Cynomolgus Monkey Cell (from whole blood)
      Specification
      from whole blood
      Preparation
      Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA 1%
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Username

      Abcam user community

      Verified customer

      Submitted Jan 02 2013

      Thank you for contacting Abcam.
      All of the antibodies that you mentioned and all the antibodies in our catalogue are guaranteed for 6 months under our Abpromise. In terms of stability, they should all have be the same, regardless of the BSA concen...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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