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ab846 has been referenced in 4 publications.
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ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Jill Montgomery
ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22ºC. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4ºC. Ab 6785 (1/800) was used as the secondary antibody
This image is courtesy of an Abreview submitted by Dr. Sandra Peternel
ab846 staining CD4 in human lymphocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab846 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/100 dilution.Double stained with Anti-CD3 antibody [PS1] (ab699), secondary Texas Red.
Image courtesy of an anonymous Abreview.
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