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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
(enlarge)


Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
(enlarge)


Product Name 

CD4 antibody [BC/1F6]

 

See all CD4 antibodies (101)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [BC/1F6] to CD4

Immunogen 

Recombinant fragment (Human) corresponding to the external domain

Reacts with 
(species key)

Hu, Bb, Mk

Tested applications 
(see key)

IHC-Fr, IHC-P


Abreviews 

Customer reviews
(see key)


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• IHC-P

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IHC-P
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IHC-P
(read Abreview)


IHC-P
(read Abreview)


Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 1-2 hours at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
Heat mediated antigen retrieval of formalin fixed paraffin embedded tissues may be required before immunostaining.
Use peroxidase block after the primary antibody or staining will be diminished.
Post-fixation with 10% formalin after the primary antibody may improve antibody staining.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil or T-cell lymphoma

Cellular localization 

Cell membrane; Single pass type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD

Relevance 

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

 

Alternative names CD4 antibody [BC/1F6] (ab846)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

920    (Human)

Omim

    

186940    (Human)

SwissProt

    

P01730    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BC/1F6

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Protein Carrier, Van Gogh Yellow Diluent, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.025 mg/ml

Storage instructions 

Store at +4°C.

Notes 

This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD4 antibody [BC/1F6] is on this datasheet. But please do contact us if you would like any reassurance!


See below for CD4 antibody [BC/1F6] images, references, products related to ab846 and other tools.

 

CD4 antibody [BC/1F6] images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Miss Jill Montgomery

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody

This image is courtesy of an Abreview submitted by Dr. Sandra Peternel

See Abreview

 

References for CD4 antibody [BC/1F6] (ab846)

Specific publication references   This product has been used in: (two most recent references)

Anraku M et al. Impact of tumor-infiltrating T cells on survival in patients with malignant pleural mesothelioma. J Thorac Cardiovasc Surg 135:823-9 (2008). PubMed: 18374762

Izban KF et al. Immunohistochemical analysis of mycosis fungoides on paraffin-embedded tissue sections. Mod Pathol 11:978-82 (1998). PubMed: 9796726
If you publish research using ab846 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to CD4

 

CD4 antibody [BC/1F6] - more information

Customer reviews (feedback) regarding CD4 antibody [BC/1F6]

Customer FAQs regarding CD4 antibody [BC/1F6]

Protocols for CD4 antibody [BC/1F6]

Price and availability of products related to CD4 antibody [BC/1F6]

 

CD4 antibody [BC/1F6] - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

 

Unmasking reagent for CD4 antibody [BC/1F6]

ab972

  

Heat Mediated High pH Antigen Retrieving Solution

 

Compatible Secondaries for CD4 antibody [BC/1F6]

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other CD4 Antibodies

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CD4 antibody [BC/1F6] - other tools:

Contact Abcam with a Technical Enquiry about CD4 antibody [BC/1F6]

 

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