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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/ab846_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-4.jpg)
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Product Name
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CD4 antibody [BC/1F6]
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See all CD4 antibodies (101)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [BC/1F6] to CD4
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Immunogen
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Recombinant fragment (Human) corresponding to the external domain
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Reacts with
(species key)
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Hu, Bb, Mk
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Tested applications
(see key)
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 1-2 hours at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
Heat mediated antigen retrieval of formalin fixed paraffin embedded tissues may be required before immunostaining.
Use peroxidase block after the primary antibody or staining will be diminished.
Post-fixation with 10% formalin after the primary antibody may improve antibody staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tonsil or T-cell lymphoma
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Cellular localization
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Cell membrane; Single pass type I membrane protein.
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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BC/1F6
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Protein Carrier, Van Gogh Yellow Diluent, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.025 mg/ml
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Storage instructions
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Store at +4°C.
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Notes
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This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD4 antibody [BC/1F6] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD4 antibody [BC/1F6] images, references, products related to ab846 and other tools.
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CD4 antibody [BC/1F6] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary. This image is courtesy of an Abreview submitted by Miss Jill Montgomery
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody This image is courtesy of an Abreview submitted by Dr. Sandra Peternel
See Abreview
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References for CD4 antibody [BC/1F6] (ab846)
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This product has been used in: (two most recent references)
Anraku M et al. Impact of tumor-infiltrating T cells on survival in patients with malignant pleural mesothelioma. J Thorac Cardiovasc Surg 135:823-9 (2008). PubMed: 18374762
Izban KF et al. Immunohistochemical analysis of mycosis fungoides on paraffin-embedded tissue sections. Mod Pathol 11:978-82 (1998). PubMed: 9796726
If you publish research using ab846 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD4
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CD4 antibody [BC/1F6] - more information
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Customer reviews (feedback) regarding CD4 antibody [BC/1F6] |
Customer FAQs regarding CD4 antibody [BC/1F6] |
Protocols for CD4 antibody [BC/1F6] |
Price and availability of products related to CD4 antibody [BC/1F6] |
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CD4 antibody [BC/1F6] - related products:
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CD4 antibody [BC/1F6] - other tools:
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Contact Abcam with a Technical Enquiry about CD4 antibody [BC/1F6] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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