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Read our guarantee »Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Anti-CD4 antibody [BC/1F6]
See all CD4 products (97) ...
Mouse monoclonal [BC/1F6] to CD4
ICC/IF, IHC-P, IHC-Frmore details
Reacts with
Human, Monkey, Baboon
Recombinant fragment (Human) corresponding to the external domain
Tonsil or T-cell lymphoma
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: Protein Carrier, Van Gogh Yellow Diluent, pH 7.3
Tissue culture supernatant
Monoclonal
BC/1F6
unknown
IgG1
unknown
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
(enlarge)
Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [BC/1F6] (ab846)
(enlarge)
Our Abpromise guarantee covers the use of ab846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(High pH- such as 9 is recommended. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method. We suggest an incubation period of 1-2 hours at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. Use peroxidase block after the primary antibody or staining will be diminished!)
IHC-Fr: Use at an assay dependent dilution. (Use peroxidase block after the primary antibody or staining will be diminished. Post-fixation with 10% formalin after the primary antibody may improve antibody staining.)
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/Images/0/ab846/ab846_1.jpg)
ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Jill Montgomery
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-4.jpg)
ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody
This image is courtesy of an Abreview submitted by Dr. Sandra Peternel
Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [BC/1F6] (ab846)
![Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-15.jpg)
ab846 staining CD4 in human lymphocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab846 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/100 dilution.Double stained with Anti-CD3 antibody [PS1] (ab699), secondary Texas Red.
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See all 4 publications for this product
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/Images/0/ab846/ab846_1.jpg)
ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Jill Montgomery
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-4.jpg)
ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody
This image is courtesy of an Abreview submitted by Dr. Sandra Peternel
![Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [BC/1F6] (ab846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-15.jpg)
ab846 staining CD4 in human lymphocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab846 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/100 dilution.Double stained with Anti-CD3 antibody [PS1] (ab699), secondary Texas Red.
Image courtesy of an anonymous Abreview.
![CD4 antibody [BC/1F6] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Monkey (846)](/ps/datasheet/images/0/ab846/CD4-Primary-antibodies-ab846-6.jpg)
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