Anti-CD4 antibody [BC/1F6] (ab846)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Miss Jill Montgomery

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [BC/1F6] (ab846)

ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody

This image is courtesy of an Abreview submitted by Dr. Sandra Peternel

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  Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [BC/1F6] (ab846)

ab846 staining CD4 in human lymphocytes by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in methanol, blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab846 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/100 dilution.

Double stained with Anti-CD3 antibody [PS1] (ab699), secondary Texas Red.

Image courtesy of an anonymous Abreview.

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