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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Chicken peripheral blood mononuclear cells were double stained with ab25420 at 0.1 µg/106 cells and a FITC-conjugated mouse anti-chicken CD3 (Clone CT-3) ab24895. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
ab25420 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"