Overview

  • Product nameAnti-CD4 antibody [CT-4]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [CT-4] to CD4
  • Specificityab25345 recognises Chicken CD4.
  • Tested applicationsFunctional Studies, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Chicken, Turkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    ab25345 inhibits both PHA- and Con A-induced proliferative responses of splenocytes, and PWM-induced IL-2 production.

Properties

Applications

Our Abpromise guarantee covers the use of ab25345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Flow Cyt
IHC-Fr
IP
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    FuncS (Inhibition of mitogen-induced proliferation): Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 Antigen antibody
      • CD4 molecule antibody
      • CD4 Receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T Cell Antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T Cell Surface Antigen T4/Leu3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T Cell Surface Glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References for Anti-CD4 antibody [CT-4] (ab25345)

    This product has been referenced in:
    • Young JA & Jefferies W Towards the conservation of endangered avian species: a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One 8:e67137 (2013). Quail . Read more (PubMed: 23825633) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some op...

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    Thank you for providing that extra information.

    We have previously used the Anti-CD4 antibody [CT-4] (ab25345) in flow cytometry at a concentration of 0.1 ug per 106 cells. Meanwhile the secondary antibody ab97239 can be used in a range of 1/...

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    Thank you for contacting us.

    1. Yes, a substrate is needed.

    2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
    > IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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    1. yes,you need a secondary antibody such as ab97239 (Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody). The reason isthat the primary antibodies you want to use (ab25345, ab25346 andab25347) are not conjugated.

    2. a.Yes, ab98693 (G...

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    Yes,the chosen antibodies are suitable for Flow Cytometry and chicken samples.

    No, none of the chosen primary antibodies (ab25345, ab25346, ab25347) are conjugated, so you need a secondary antibody such as ab97239.

    The secondary ant...

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    Thank you for contacting us.

    I am happy to confirm that your chosen antibodies are suitable in Flow Cytometry and chicken, and that nothing else should be required.

    However, I am wondering about the usage of the ab37382 Chicken IgY...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"