• Product nameAnti-CD4 antibody [CT-4]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [CT-4] to CD4
  • Specificityab25345 recognises Chicken CD4.
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Chicken, Turkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    ab25345 inhibits both PHA- and Con A-induced proliferative responses of splenocytes, and PWM-induced IL-2 production.



Our Abpromise guarantee covers the use of ab25345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Inhibition of mitogen-induced proliferation.

Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References for Anti-CD4 antibody [CT-4] (ab25345)

This product has been referenced in:
  • Young JA & Jefferies W Towards the conservation of endangered avian species: a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One 8:e67137 (2013). Quail . Read more (PubMed: 23825633) »

See 1 Publication for this product

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Thank you for contacting us.

The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some op...

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Thank you for providing that extra information.

We have previously used the Anti-CD4 antibody [CT-4] (ab25345) in flow cytometry at a concentration of 0.1 ug per 106 cells. Meanwhile the secondary antibody ab97239 can be used in a range of 1/...

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Thank you for contacting us.

1. Yes, a substrate is needed.

2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
> IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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1. yes,you need a secondary antibody such as ab97239 (Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody). The reason isthat the primary antibodies you want to use (ab25345, ab25346 andab25347) are not conjugated.

2. a.Yes, ab98693 (G...

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Yes,the chosen antibodies are suitable for Flow Cytometry and chicken samples.

No, none of the chosen primary antibodies (ab25345, ab25346, ab25347) are conjugated, so you need a secondary antibody such as ab97239.

The secondary ant...

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Thank you for contacting us.

I am happy to confirm that your chosen antibodies are suitable in Flow Cytometry and chicken, and that nothing else should be required.

However, I am wondering about the usage of the ab37382 Chicken IgY...

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